: LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode.Anandamide (N-arachidonoylethanolamine, AEA) and 2-Arachidonoylglycerol (2-AG) are the most abundant endocannabinoids (eCBs), which play a major role in a wide variety of physiological and pathological processes. Analysis of those compounds by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine AEA and 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy-to-perform extraction procedures, with reduced solvent consumption.

LC-MS/MS Analysis of AEA and 2-AG / Battista, Natalia; Fanti, Federico; Sergi, Manuel. - (2023), pp. 41-47. [10.1007/978-1-0716-2728-0_4].

LC-MS/MS Analysis of AEA and 2-AG

Sergi, Manuel
2023

Abstract

: LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode.Anandamide (N-arachidonoylethanolamine, AEA) and 2-Arachidonoylglycerol (2-AG) are the most abundant endocannabinoids (eCBs), which play a major role in a wide variety of physiological and pathological processes. Analysis of those compounds by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine AEA and 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy-to-perform extraction procedures, with reduced solvent consumption.
2023
LC–MS/MS Analysis of AEA and 2-AG
978-1-0716-2727-3
978-1-0716-2728-0
2-AG; AEA; LC–MS/MS; Plasma; Tissue; μSPE
02 Pubblicazione su volume::02a Capitolo o Articolo
LC-MS/MS Analysis of AEA and 2-AG / Battista, Natalia; Fanti, Federico; Sergi, Manuel. - (2023), pp. 41-47. [10.1007/978-1-0716-2728-0_4].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1696299
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