Background Several factors can affect wound healing 1 , bacterial infection, above all, significantly contributing to the persistence of chronic wounds. In this context, wound dressings are considered essential devices since they can play an active role in tissue regeneration and wound closure 2 . Methods Antimicrobial 2D-matrices based on sodium alginate (Alg), citric acid (CA, 9 mg/cm 2 ) and xylitol (Xy) at different contents (10, 20, 30, 40% w/w) were prepared using solvent casting method and characterized for application as wound dressings. Antimicrobial and antibiofilm activities were evaluated against four bacterial pathogens, 2 Gram positives and 2 Gram negatives. Results and discussions CA significantly improved water stability of the Alg 2D-matrixes presumably because of its ability to establish multipoint interactions with the matrix. Xy increased film ductility (elongation to break up to 35-45 %) and transparency while decreased water vapour transmission rate in a concentration-dependent manner. AlgCA matrix showed a good in vitro antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis (inhibition halo of 12, 14, 26 and 8 mm, respectively). Moreover, a potential synergistic action between Xy and AC was observed in AlgXy30 matrix (containing Xy at 30% and CA at 9 mg/cm 2 ) towards P. aeruginosa and S. aureus. Conclusions Among the developed alginate-based 2D-matrices, AlgXy30 exhibited the best physico- chemical and antimicrobial properties. The found synergistic activity between CA and Xy opens interesting perspectives in the development of wound dressings minimizing the risk of drug resistance. [1] G. A. James, E. Swogger, R. Wolcott, E. d. Pulcini, P. Secor, J. Sestrich, J. W. Costerton e P. S. Stewart. Biofilms in chronic wounds. Wound Repair and Regeneration, n.16, pp. 37-44, 2008. [2] Gianino E, Miller C, Gilmore J. Smart Wound Dressings for Diabetic Chronic Wounds. Bioengineering. 2018; 5(3):51.
Antimicrobial 2D-matrices based on alginate as wound dressings / Brugnoli, Benedetta; Vuotto, Claudia; Piozzi, Antonella; Francolini, Iolanda. - (2022). (Intervento presentato al convegno EPNOE Workshop, Polysaccharides in Drug Delivery tenutosi a Sapienza University of Rome).
Antimicrobial 2D-matrices based on alginate as wound dressings
Benedetta Brugnoli;Antonella Piozzi;Iolanda Francolini
2022
Abstract
Background Several factors can affect wound healing 1 , bacterial infection, above all, significantly contributing to the persistence of chronic wounds. In this context, wound dressings are considered essential devices since they can play an active role in tissue regeneration and wound closure 2 . Methods Antimicrobial 2D-matrices based on sodium alginate (Alg), citric acid (CA, 9 mg/cm 2 ) and xylitol (Xy) at different contents (10, 20, 30, 40% w/w) were prepared using solvent casting method and characterized for application as wound dressings. Antimicrobial and antibiofilm activities were evaluated against four bacterial pathogens, 2 Gram positives and 2 Gram negatives. Results and discussions CA significantly improved water stability of the Alg 2D-matrixes presumably because of its ability to establish multipoint interactions with the matrix. Xy increased film ductility (elongation to break up to 35-45 %) and transparency while decreased water vapour transmission rate in a concentration-dependent manner. AlgCA matrix showed a good in vitro antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis (inhibition halo of 12, 14, 26 and 8 mm, respectively). Moreover, a potential synergistic action between Xy and AC was observed in AlgXy30 matrix (containing Xy at 30% and CA at 9 mg/cm 2 ) towards P. aeruginosa and S. aureus. Conclusions Among the developed alginate-based 2D-matrices, AlgXy30 exhibited the best physico- chemical and antimicrobial properties. The found synergistic activity between CA and Xy opens interesting perspectives in the development of wound dressings minimizing the risk of drug resistance. [1] G. A. James, E. Swogger, R. Wolcott, E. d. Pulcini, P. Secor, J. Sestrich, J. W. Costerton e P. S. Stewart. Biofilms in chronic wounds. Wound Repair and Regeneration, n.16, pp. 37-44, 2008. [2] Gianino E, Miller C, Gilmore J. Smart Wound Dressings for Diabetic Chronic Wounds. Bioengineering. 2018; 5(3):51.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
