Target gene silencing is a strategy that can be used to turn off pathways or genes which are difficult to turn off pharmacologically, both because of lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect primary cell cultures.
Methods for modulating the pathway of NF-κB using short hairpin RNA (ShRNA) / Moretti, M.; Di Francesco, B.; Nolfi, M. D. V.; Angrisani, A.; De Smaele, E.. - (2021), pp. 95-107. - METHODS IN MOLECULAR BIOLOGY. [10.1007/978-1-0716-1669-7_6].
Methods for modulating the pathway of NF-κB using short hairpin RNA (ShRNA)
Moretti M.Primo
;Angrisani A.;De Smaele E.Ultimo
2021
Abstract
Target gene silencing is a strategy that can be used to turn off pathways or genes which are difficult to turn off pharmacologically, both because of lack of targeting drugs, or because of the risk of wider off-target effects. Here we describe the design and use of short hairpin RNA (ShRNA) and lentiviral vectors as an efficient technique for silencing NF-kappaB (NF-κB) pathway in cultured cells. This method can be used also in hard to transfect primary cell cultures.| File | Dimensione | Formato | |
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