Metastatic melanoma is one of the most aggressive types of cancers, diffused worldwide and with a significant percentage of lethality. The employment of animal models to test therapeutic strategies against melanoma growth and metastatic spread is of key relevance for cancer biologists. In this regard, the count of metastatic foci in murine lung tissue is one of the recognized methods to monitor macrometastases of melanoma. Here, we illustrate a clonogenic assay method to detect with high sensitivity the presence of single melanoma cells (micrometastases) at the pulmonary level when metastatic foci are still not detectable in the tissue. This method allows for high precision detection and quantification of melanoma metastatic spread to the lung at early stages.

A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue / Mattei, F.; Andreone, S.; Schiavoni, G.. - (2021), pp. 385-406. - METHODS IN MOLECULAR BIOLOGY. [10.1007/978-1-0716-1205-7_28].

A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue

Andreone S.;
2021

Abstract

Metastatic melanoma is one of the most aggressive types of cancers, diffused worldwide and with a significant percentage of lethality. The employment of animal models to test therapeutic strategies against melanoma growth and metastatic spread is of key relevance for cancer biologists. In this regard, the count of metastatic foci in murine lung tissue is one of the recognized methods to monitor macrometastases of melanoma. Here, we illustrate a clonogenic assay method to detect with high sensitivity the presence of single melanoma cells (micrometastases) at the pulmonary level when metastatic foci are still not detectable in the tissue. This method allows for high precision detection and quantification of melanoma metastatic spread to the lung at early stages.
2021
Methods in Molecular Biology
978-1-0716-1204-0
978-1-0716-1205-7
B16.F10; Clonogenic assay; Crystal Violet; ImageJ macros; Melanoma; Micrometastasis; Mouse model; R scripts; Animals; Cell Line, Tumor; Lung Neoplasms; Melanoma, Experimental; Mice; Neoplasm Metastasis; Tumor Stem Cell Assay
02 Pubblicazione su volume::02a Capitolo o Articolo
A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue / Mattei, F.; Andreone, S.; Schiavoni, G.. - (2021), pp. 385-406. - METHODS IN MOLECULAR BIOLOGY. [10.1007/978-1-0716-1205-7_28].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1529300
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