Considerable strides have been made in understanding the oxidative mechanisms involved in the final steps of the cysteine pathway leading to taurine. The oxidation of sulfinates, hypotaurine and cysteine sulfinic acid, to the respective sulfonates, taurine and cysteic acid, has never been associated with any specific enzyme. Conversely, there is strong evidence that in vivo formation of taurine and cysteic acid is the result of sulfinate interaction with a variety of biologically relevant oxidants. In the last decade, many experiments have been performed to understand whether peroxynitrite, nitrogen dioxide and carbonate radical anion could be included in the biologically relevant reactive species capable of oxidizing sulfinates. Thanks to this work, it has been possible to highlight two possible reaction mechanisms (direct and indirect reaction) of sulfinates with reactive oxygen and nitrogen species.The sulfinates oxidation, mediated by peroxynitrite, is an example of both reaction mechanisms: through a two-electron-direct-reaction with peroxynitrite or through a one-electron-indirect-transfer reaction. In the indirect mechanism, the peroxynitrite homolysis releases hydroxyl and nitrogen dioxide radical and in addition the degradation of short-lived adduct formed by peroxynitrite and CO2 can generate carbonate radical anion. The reaction of hypotaurine and cysteine sulfinic acid with peroxynitrite-derived radicals is accompanied by extensive oxygen uptake with the generation of transient intermediates, which can begin a reaction by an oxygen-dependent mechanism with the sulfonates, taurine, and cysteic acid as final products. Due to pulse radiolysis studies, it has been shown that transient sulfonyl radicals (RSO2(•)) have been produced during the oxidation of both sulfinates by one-electron transfer reaction.The purpose is to analyze all the aspects of the reactive mechanism in the sulfinic group oxidation of hypotaurine and cysteine sulfinic acid through the results obtained from our laboratory in recent years.
The interaction of hypotaurine and other sulfinates with reactive oxygen and nitrogen species: a survey of reaction mechanisms / BASEGGIO CONRADO, Alessia; D'Angelantonio, Mila; D'Erme, Maria; Pecci, Laura; Fontana, Mario. - STAMPA. - 975(2017), pp. 573-583.
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|Titolo:||The interaction of hypotaurine and other sulfinates with reactive oxygen and nitrogen species: a survey of reaction mechanisms|
FONTANA, Mario (Corresponding author)
|Data di pubblicazione:||2017|
|Citazione:||The interaction of hypotaurine and other sulfinates with reactive oxygen and nitrogen species: a survey of reaction mechanisms / BASEGGIO CONRADO, Alessia; D'Angelantonio, Mila; D'Erme, Maria; Pecci, Laura; Fontana, Mario. - STAMPA. - 975(2017), pp. 573-583.|
|Appartiene alla tipologia:||02a Capitolo o Articolo|