Background Assessing vitamin E status in humans is critical for nutritional evaluation and verification of clinical and biological compliance of supplemented subjects. An accurate analytical method for measuring the two main vitamin E isoforms, i.e. α- and γ-tocopherol (α- and γ-TOH) in small volumes of plasma can facilitate the application of this analysis to clinical trials and in situations where a limited amount of sample is available. Methods We have developed a micro method, which uses only 5 μL plasma, based on isotope dilution, trimethylsilation and GC-MS. The method was validated according to the guidelines of the International Conference on Harmonization of analytical procedures. The method was also applied to 5 μL of whole blood for the potential use in conditions were the availability of specimens is limited. Results Accurate quantitation of α-TOH and γ-TOH was achieved at levels ≥ 0.417 μM and ≥ 0,007 μM, respectively. Within-day coefficient of variation was 1.31 % and 4
Gas chromatography-mass spectrometry microanalysis of alpha- and gamma-tocopherol in plasma and whole blood / Zerbinati, Chiara; Galli, F; Regolanti, Romolo; Poli, G; Iuliano, Luigi. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 446:(2015), pp. 156-162. [10.1016/j.cca.2015.04.026]
Gas chromatography-mass spectrometry microanalysis of alpha- and gamma-tocopherol in plasma and whole blood
ZERBINATI, CHIARA;REGOLANTI, ROMOLO;IULIANO, Luigi
2015
Abstract
Background Assessing vitamin E status in humans is critical for nutritional evaluation and verification of clinical and biological compliance of supplemented subjects. An accurate analytical method for measuring the two main vitamin E isoforms, i.e. α- and γ-tocopherol (α- and γ-TOH) in small volumes of plasma can facilitate the application of this analysis to clinical trials and in situations where a limited amount of sample is available. Methods We have developed a micro method, which uses only 5 μL plasma, based on isotope dilution, trimethylsilation and GC-MS. The method was validated according to the guidelines of the International Conference on Harmonization of analytical procedures. The method was also applied to 5 μL of whole blood for the potential use in conditions were the availability of specimens is limited. Results Accurate quantitation of α-TOH and γ-TOH was achieved at levels ≥ 0.417 μM and ≥ 0,007 μM, respectively. Within-day coefficient of variation was 1.31 % and 4File | Dimensione | Formato | |
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