With the aim of exploring the potential of ultra-​fast chiral chromatog. for high-​throughput anal., the new sub-​2 μ Whelk-​O 1 chiral stationary phase (CSP) has been employed in supercrit. fluid conditions to screen 129 racemates, mainly of pharmaceutical interest.By using a 5-​cm long column (0.46 cm internal diam.)​, a single co-​solvent (MeOH) and a 7-​min gradient elution, 85​% of acidic and neutral analytes considered in this work have been successfully resolved, with resoln. (Rs) larger than 2 in more than 65​% of cases. Moreover, almost a half of basic samples that, for their own characteristics, are known to be difficult to sep. on Whelk-​O 1 CSP, have shown Rs greater than 0.3. The screening of the entire library could be accomplished in less than 24 h (single run) with 63​% of pos. score.For well-​resolved enantiomers (Rs roughly included between 1 and 3)​, we show that method transfer from gradient to isocratic conditions is straightforward. In many cases, isocratic ultra-​fast sepns. (with anal. time smaller than 60 s) have been achieved by simply employing, as isocratic mobile phase, the eluent compn. at which the second enantiomer was eluted in gradient mode.By considering the extension and variety of the library in terms of chemico-​phys. and structural properties of compds. and numerousness, we believe that this work demonstrates the real potential of the technique for high-​throughput enantioselective screening.

Expanding the potential of chiral chromatography for high-throughput screening of large compound libraries by means of sub–2μ Whelk-O 1 stationary phase in supercritical fluid conditions / Sciascera, Luca; Ismail, OMAR HABIB; Ciogli, Alessia; Kotoni, Dorina; Cavazzini, Alberto; Botta, Lorenzo; Szczerba, Ted; Kocergin, Jelena; Villani, Claudio; Gasparrini, Francesco. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - STAMPA. - 1383:(2015), pp. 160-168. [10.1016/j.chroma.2015.01.042]

Expanding the potential of chiral chromatography for high-throughput screening of large compound libraries by means of sub–2μ Whelk-O 1 stationary phase in supercritical fluid conditions

SCIASCERA, LUCA;ISMAIL, OMAR HABIB;CIOGLI, Alessia
;
KOTONI, DORINA;VILLANI, Claudio;GASPARRINI, Francesco
2015

Abstract

With the aim of exploring the potential of ultra-​fast chiral chromatog. for high-​throughput anal., the new sub-​2 μ Whelk-​O 1 chiral stationary phase (CSP) has been employed in supercrit. fluid conditions to screen 129 racemates, mainly of pharmaceutical interest.By using a 5-​cm long column (0.46 cm internal diam.)​, a single co-​solvent (MeOH) and a 7-​min gradient elution, 85​% of acidic and neutral analytes considered in this work have been successfully resolved, with resoln. (Rs) larger than 2 in more than 65​% of cases. Moreover, almost a half of basic samples that, for their own characteristics, are known to be difficult to sep. on Whelk-​O 1 CSP, have shown Rs greater than 0.3. The screening of the entire library could be accomplished in less than 24 h (single run) with 63​% of pos. score.For well-​resolved enantiomers (Rs roughly included between 1 and 3)​, we show that method transfer from gradient to isocratic conditions is straightforward. In many cases, isocratic ultra-​fast sepns. (with anal. time smaller than 60 s) have been achieved by simply employing, as isocratic mobile phase, the eluent compn. at which the second enantiomer was eluted in gradient mode.By considering the extension and variety of the library in terms of chemico-​phys. and structural properties of compds. and numerousness, we believe that this work demonstrates the real potential of the technique for high-​throughput enantioselective screening.
2015
enantioselective high throughput screening; Sub-2μm Whelk-O 1; ultra-fast chiral separations; eUHPSFC
01 Pubblicazione su rivista::01a Articolo in rivista
Expanding the potential of chiral chromatography for high-throughput screening of large compound libraries by means of sub–2μ Whelk-O 1 stationary phase in supercritical fluid conditions / Sciascera, Luca; Ismail, OMAR HABIB; Ciogli, Alessia; Kotoni, Dorina; Cavazzini, Alberto; Botta, Lorenzo; Szczerba, Ted; Kocergin, Jelena; Villani, Claudio; Gasparrini, Francesco. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - STAMPA. - 1383:(2015), pp. 160-168. [10.1016/j.chroma.2015.01.042]
File allegati a questo prodotto
File Dimensione Formato  
Sciascera_Expanding-the potential_2015.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.47 MB
Formato Adobe PDF
1.47 MB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/759665
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 47
  • ???jsp.display-item.citation.isi??? 42
social impact