Electrophysiological, biochemical, and Ca2+ imaging studies of cultured mouse myotubes were used to investigate whether the neurotransmitter acetylcholine causes an increase in intracellular Ca2+ concentration ([Ca2+]i) through activation of a second messenger system. Bath applications of acetylcholine to myotubes (i) elicited a significant membrane current even in a Na+-free Ca2+ medium, when the current was carried mainly by calcium ions; (ii) caused a rapid and transient cytosolic accumulation of inositol 1,4,5-trisphosphate; (iii) evoked a conspicuous alpha-bungarotoxin-sensitive long-lasting [Ca2+]i enhancement even in the presence of Cd2+; and (iv) transiently increased [Ca2+]i when cells were equilibrated in a Ca2+-free atropine-containing medium. We propose that, in addition to opening ion channels, the nicotinic action of acetylcholine on the muscle cell membrane increases [Ca2+]i through activation of the inositol 1,4,5-trisphosphate second messenger system and mobilization of Ca2+ from intracellular stores.

ACETYLCHOLINE INDUCES VOLTAGE-INDEPENDENT INCREASE OF CYTOSOLIC CALCIUM IN MOUSE MYOTUBES / A., Giovanelli; Grassi, Francesca; E., Mattei; A. M., Mileo; Eusebi, Fabrizio. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 88:22(1991), pp. 10069-10073. [10.1073/pnas.88.22.10069]

ACETYLCHOLINE INDUCES VOLTAGE-INDEPENDENT INCREASE OF CYTOSOLIC CALCIUM IN MOUSE MYOTUBES

GRASSI, Francesca;EUSEBI, Fabrizio
1991

Abstract

Electrophysiological, biochemical, and Ca2+ imaging studies of cultured mouse myotubes were used to investigate whether the neurotransmitter acetylcholine causes an increase in intracellular Ca2+ concentration ([Ca2+]i) through activation of a second messenger system. Bath applications of acetylcholine to myotubes (i) elicited a significant membrane current even in a Na+-free Ca2+ medium, when the current was carried mainly by calcium ions; (ii) caused a rapid and transient cytosolic accumulation of inositol 1,4,5-trisphosphate; (iii) evoked a conspicuous alpha-bungarotoxin-sensitive long-lasting [Ca2+]i enhancement even in the presence of Cd2+; and (iv) transiently increased [Ca2+]i when cells were equilibrated in a Ca2+-free atropine-containing medium. We propose that, in addition to opening ion channels, the nicotinic action of acetylcholine on the muscle cell membrane increases [Ca2+]i through activation of the inositol 1,4,5-trisphosphate second messenger system and mobilization of Ca2+ from intracellular stores.
1991
acetylcholine receptor; acetylcholine receptors; acetylchollne receptors; ca2+ transients; calcium mobilization; electrophysiology; inositol 1; 4; 5-triphosphate; 5-trisphosphate
01 Pubblicazione su rivista::01a Articolo in rivista
ACETYLCHOLINE INDUCES VOLTAGE-INDEPENDENT INCREASE OF CYTOSOLIC CALCIUM IN MOUSE MYOTUBES / A., Giovanelli; Grassi, Francesca; E., Mattei; A. M., Mileo; Eusebi, Fabrizio. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 88:22(1991), pp. 10069-10073. [10.1073/pnas.88.22.10069]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/74051
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