This review summarizes all the research efforts in the last decade (1994-2003) that have been spent to the various application of immobilized enzyme reactor (IMER) in on-line high performance liquid chromatography (HPLC). All immobilization procedures including supports, kind of assembly into chromatographic system and methods are described. The effect of immobilization on enzymatic properties and stability of biocatalysts is considered. A brief survey of the main applications of IMER both as pre-column, post-column or column in the chemical, pharmaceutical, clinical and commodities fields is also reported. (c) 2005 Elsevier B.V. All rights reserved.
Application of immobilized enzyme reactor in on-line high performance liquid chromatography: A review / Girelli, Anna Maria; Enrico, Mattei. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - STAMPA. - 819:1(2005), pp. 3-16. [10.1016/j.jchromb.2005.01.031]
Application of immobilized enzyme reactor in on-line high performance liquid chromatography: A review
GIRELLI, Anna Maria;
2005
Abstract
This review summarizes all the research efforts in the last decade (1994-2003) that have been spent to the various application of immobilized enzyme reactor (IMER) in on-line high performance liquid chromatography (HPLC). All immobilization procedures including supports, kind of assembly into chromatographic system and methods are described. The effect of immobilization on enzymatic properties and stability of biocatalysts is considered. A brief survey of the main applications of IMER both as pre-column, post-column or column in the chemical, pharmaceutical, clinical and commodities fields is also reported. (c) 2005 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
