Genetic transformation is defined as a process of introducing genetic material (i.e. DNA) into a cell that results in a heritable change. Many approaches now exist that allow routine transformation. Although the biolistic procedure is not the most widely applied transformation technique, it is a key method to modify the mitochondrial DNA and is an advisable method for gene transfer into various organisms and tissues that have proved recalcitrant to more conventional means. This method is widely used for Saccharomyces cerevisiae mitochondrial transformation. We describe here the methodology as well as the most important results which were obtained.

Biolistic Transformation for Delivering DNA into the Mitochondria / Montanari, Arianna; M., Bolotin Fukuhara; M., Fazzi D’Orsi; DE LUCA, Cristina; Bianchi, Michele Maria; Francisci, Silvia. - STAMPA. - (2015), pp. 101-117. [10.1007/978-3-319-10142-2_10].

Biolistic Transformation for Delivering DNA into the Mitochondria

MONTANARI, Arianna;DE LUCA, CRISTINA;BIANCHI, Michele Maria;FRANCISCI, Silvia
2015

Abstract

Genetic transformation is defined as a process of introducing genetic material (i.e. DNA) into a cell that results in a heritable change. Many approaches now exist that allow routine transformation. Although the biolistic procedure is not the most widely applied transformation technique, it is a key method to modify the mitochondrial DNA and is an advisable method for gene transfer into various organisms and tissues that have proved recalcitrant to more conventional means. This method is widely used for Saccharomyces cerevisiae mitochondrial transformation. We describe here the methodology as well as the most important results which were obtained.
2015
Genetic Transformation Systems in Fungi
978-3-319-10141-5
978-3-319-10142-2
Transformation; mitochondria; yeast
02 Pubblicazione su volume::02a Capitolo o Articolo
Biolistic Transformation for Delivering DNA into the Mitochondria / Montanari, Arianna; M., Bolotin Fukuhara; M., Fazzi D’Orsi; DE LUCA, Cristina; Bianchi, Michele Maria; Francisci, Silvia. - STAMPA. - (2015), pp. 101-117. [10.1007/978-3-319-10142-2_10].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/552673
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