This protocol describes formaldehyde fixation of Drosophila testes. The procedure preserves chromosome morphology very well, allowing a clear visualization of the chromosome condensation-decondensation cycle. It also results in excellent preservation of microtubules for immunostaining. The main disadvantage of this fixation technique is its poor preservation of cell morphology, as viewed by phase-contrast optics. In addition, because it involves a rather hard squashing of formaldehyde-fixed testes, it results in meiotic figures which are flatter and larger than those obtained using methanol-acetone or paraformaldehyde fixation protocols.
Formaldehyde fixation of Drosophila testes / Bonaccorsi, Silvia; M. G., Giansanti; Cenci, Giovanni; M., Gatti. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2012:8(2012), pp. pdb.prot067355-pdb.prot067355. [10.1101/pdb.prot067355]
Formaldehyde fixation of Drosophila testes.
BONACCORSI, Silvia;CENCI, GIOVANNI;
2012
Abstract
This protocol describes formaldehyde fixation of Drosophila testes. The procedure preserves chromosome morphology very well, allowing a clear visualization of the chromosome condensation-decondensation cycle. It also results in excellent preservation of microtubules for immunostaining. The main disadvantage of this fixation technique is its poor preservation of cell morphology, as viewed by phase-contrast optics. In addition, because it involves a rather hard squashing of formaldehyde-fixed testes, it results in meiotic figures which are flatter and larger than those obtained using methanol-acetone or paraformaldehyde fixation protocols.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.