This protocol describes formaldehyde fixation of Drosophila testes. The procedure preserves chromosome morphology very well, allowing a clear visualization of the chromosome condensation-decondensation cycle. It also results in excellent preservation of microtubules for immunostaining. The main disadvantage of this fixation technique is its poor preservation of cell morphology, as viewed by phase-contrast optics. In addition, because it involves a rather hard squashing of formaldehyde-fixed testes, it results in meiotic figures which are flatter and larger than those obtained using methanol-acetone or paraformaldehyde fixation protocols.

Formaldehyde fixation of Drosophila testes / Bonaccorsi, Silvia; M. G., Giansanti; Cenci, Giovanni; M., Gatti. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2012:8(2012), pp. pdb.prot067355-pdb.prot067355. [10.1101/pdb.prot067355]

Formaldehyde fixation of Drosophila testes.

BONACCORSI, Silvia;CENCI, GIOVANNI;
2012

Abstract

This protocol describes formaldehyde fixation of Drosophila testes. The procedure preserves chromosome morphology very well, allowing a clear visualization of the chromosome condensation-decondensation cycle. It also results in excellent preservation of microtubules for immunostaining. The main disadvantage of this fixation technique is its poor preservation of cell morphology, as viewed by phase-contrast optics. In addition, because it involves a rather hard squashing of formaldehyde-fixed testes, it results in meiotic figures which are flatter and larger than those obtained using methanol-acetone or paraformaldehyde fixation protocols.
2012
methods; fixatives; animals; drosophila; metabolism; male; anatomy /&/ histology; formaldehyde; testis; tissue fixation; entomology
01 Pubblicazione su rivista::01a Articolo in rivista
Formaldehyde fixation of Drosophila testes / Bonaccorsi, Silvia; M. G., Giansanti; Cenci, Giovanni; M., Gatti. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2012:8(2012), pp. pdb.prot067355-pdb.prot067355. [10.1101/pdb.prot067355]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/519590
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