We have developed a technique of homologous recombination in bacteria which allows the mutagenesis of large genomic fragments cloned in cosmids. The desired mutation is first introduced into a plasmid clone and is then transferred to the appropriate cosmid clone by the means of double antibiotic selection coupled with phenotypic selection. We describe three different types of construct made by this technique.
Generation of small mutation in large genomic fragments by homologous recombination: description of the technique and examples of its use / Tripodi, Marco; Perfumo, S; Ali, R; Amicone, Laura; Abbott, C; Cortese, R.. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 18:(1990), pp. 6247-6251. [10.1093/nar/18.21.6247]
Generation of small mutation in large genomic fragments by homologous recombination: description of the technique and examples of its use.
TRIPODI, Marco;AMICONE, Laura;
1990
Abstract
We have developed a technique of homologous recombination in bacteria which allows the mutagenesis of large genomic fragments cloned in cosmids. The desired mutation is first introduced into a plasmid clone and is then transferred to the appropriate cosmid clone by the means of double antibiotic selection coupled with phenotypic selection. We describe three different types of construct made by this technique.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
