A hydrogen peroxide electrochemical sensor, coupled with two enzymes (ß -galactosidase and glucose oxidase) immobilized on the surface of the sensor covered with a cellulose acetate dialysis membrane (cut off 100), is proposed for the analysis of lactose in raw milk. The resulting hydrogen peroxide formation is measured with a platinum electrode polarized at +650 mV versus Ag/AgCl. The procedure developed is very simple and the short response time permits its use in assaying milk samples on dairy farms.
Lactose determination in raw milk with a two-enzyme based electrochemical sensor / R., Pilloton; Mascini, Marco; I. G., Casella; Festa, Maria Rosa; Bottari, Emilio Giovanni. - In: ANALYTICAL LETTERS. - ISSN 0003-2719. - STAMPA. - 20:11(1987), pp. 1803-1814. [10.1080/00032718708082597]
Lactose determination in raw milk with a two-enzyme based electrochemical sensor.
MASCINI, Marco;FESTA, Maria Rosa;BOTTARI, Emilio Giovanni
1987
Abstract
A hydrogen peroxide electrochemical sensor, coupled with two enzymes (ß -galactosidase and glucose oxidase) immobilized on the surface of the sensor covered with a cellulose acetate dialysis membrane (cut off 100), is proposed for the analysis of lactose in raw milk. The resulting hydrogen peroxide formation is measured with a platinum electrode polarized at +650 mV versus Ag/AgCl. The procedure developed is very simple and the short response time permits its use in assaying milk samples on dairy farms.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
