A nanopore test circuit is proposed for single-strand DNA sequencing, which allows real-time sensing of the electric conductance of individual sections of a DNA strand as it is pulled through the nanopore by an electric current at a controlled speed. The test circuit is based on a planar microchamber with a nanochannel drilled through its multilayer graphene electrode by an electron beam. The nanochannel is self-aligned with a nanopore created in the lipid bilayer membrane of liposomes by nanosecond electric pulses. Simulation shows that by carefully controlling the magnitude, period, and repetition rate of the pulses, the diameter of the nanopore can be optimized for the best speed the DNA is pulled through the nanopore. © 2012 IEEE.
Nanopore test circuit for single-strand DNA sequencing / C., Palego; J. c. m., Hwang; C., Merla; Apollonio, Francesca; Liberti, Micaela. - ELETTRONICO. - (2012), pp. 101-104. (Intervento presentato al convegno 2012 IEEE 12th Topical Meeting on Silicon Monolithic Integrated Circuits in RF Systems, SiRF 2012 tenutosi a Santa Clara, CA nel 16 January 2012 through 18 January 2012) [10.1109/sirf.2012.6160154].
Nanopore test circuit for single-strand DNA sequencing
APOLLONIO, Francesca;LIBERTI, Micaela
2012
Abstract
A nanopore test circuit is proposed for single-strand DNA sequencing, which allows real-time sensing of the electric conductance of individual sections of a DNA strand as it is pulled through the nanopore by an electric current at a controlled speed. The test circuit is based on a planar microchamber with a nanochannel drilled through its multilayer graphene electrode by an electron beam. The nanochannel is self-aligned with a nanopore created in the lipid bilayer membrane of liposomes by nanosecond electric pulses. Simulation shows that by carefully controlling the magnitude, period, and repetition rate of the pulses, the diameter of the nanopore can be optimized for the best speed the DNA is pulled through the nanopore. © 2012 IEEE.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
