The activation by antibody of the defective Z gene product (AMEF) of a lac-negative E.coli is being investigated with the aim of deriving a general mechanism for antibody-mediated conformational changes in proteins. The present data on kinetics of activation and on the effect of substrate analogs on the mutant beta-galacotsidase show that interaction with different ligands at specific sites of the proteins can stabiliza or induce an enzymatically active conformation similar to that of the wild type enzyme
Antibody-mediated activation of beta-galactosidase mutants and complementing fragments / Celada, F; Radojkovic, J; Strom, Roberto. - In: JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE. - ISSN 0021-7689. - STAMPA. - 71:7-8(1974), pp. 1007-1011.
Antibody-mediated activation of beta-galactosidase mutants and complementing fragments
STROM, Roberto
1974
Abstract
The activation by antibody of the defective Z gene product (AMEF) of a lac-negative E.coli is being investigated with the aim of deriving a general mechanism for antibody-mediated conformational changes in proteins. The present data on kinetics of activation and on the effect of substrate analogs on the mutant beta-galacotsidase show that interaction with different ligands at specific sites of the proteins can stabiliza or induce an enzymatically active conformation similar to that of the wild type enzymeI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
