Ciliary neurotrophic factor (CNTF) drives the sequential assembly of a receptor complex containing the ligand-specific ai-receptor subunit (CNTFRei) and the signal transducers gpl3O and leukemia inhibitory factor receptor-a (LIFR). The Dl structural motif, located at the beginning of the D-helix ofhuman CNTF, contains two amino acid residues, F152 and K155, which are conserved among all cytokines that signal through LIFR. The functional importance of these residues was assessed by alanine mutagenesis. Substitution of either F152 or K155 with alanine was found to specifically inhibit cytokine interaction with LIFR without affecting binding to CNTFRa or gpl3O. The resulting variants behaved as partial agonists with varying degrees of residual bioactivity in different cell-based assays. Simultaneous alanine substitution of both F152 and K155 totally abolished biological activity. Combining these mutations with amino acid substitutions in the D-helix, which enhance binding affinity for the CNTFRa, gave rise to a potent competitive CNTF receptor antagonist. This protein constitutes a new tool for studies of CNTF function in normal physiology and disease.

Identification of ciliary neurotrophic factor (CNTF) residues essential for leukemia inhibitory factor receptor binding and generation of CNTF receptor antagonists / A., Di Marco; I., Gloaguen; R., Graziani; G., Paonessa; Saggio, Isabella; K. R., Hudson; R., Laufer. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 93:17(1996), pp. 9247-9252. [10.1073/pnas.93.17.9247]

Identification of ciliary neurotrophic factor (CNTF) residues essential for leukemia inhibitory factor receptor binding and generation of CNTF receptor antagonists

SAGGIO, Isabella;
1996

Abstract

Ciliary neurotrophic factor (CNTF) drives the sequential assembly of a receptor complex containing the ligand-specific ai-receptor subunit (CNTFRei) and the signal transducers gpl3O and leukemia inhibitory factor receptor-a (LIFR). The Dl structural motif, located at the beginning of the D-helix ofhuman CNTF, contains two amino acid residues, F152 and K155, which are conserved among all cytokines that signal through LIFR. The functional importance of these residues was assessed by alanine mutagenesis. Substitution of either F152 or K155 with alanine was found to specifically inhibit cytokine interaction with LIFR without affecting binding to CNTFRa or gpl3O. The resulting variants behaved as partial agonists with varying degrees of residual bioactivity in different cell-based assays. Simultaneous alanine substitution of both F152 and K155 totally abolished biological activity. Combining these mutations with amino acid substitutions in the D-helix, which enhance binding affinity for the CNTFRa, gave rise to a potent competitive CNTF receptor antagonist. This protein constitutes a new tool for studies of CNTF function in normal physiology and disease.
1996
antagonist; ciliary neurotrophic factor; cntf; gene function; gp130; leukemia; leukemia inhibitory factor; lifr
01 Pubblicazione su rivista::01a Articolo in rivista
Identification of ciliary neurotrophic factor (CNTF) residues essential for leukemia inhibitory factor receptor binding and generation of CNTF receptor antagonists / A., Di Marco; I., Gloaguen; R., Graziani; G., Paonessa; Saggio, Isabella; K. R., Hudson; R., Laufer. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 93:17(1996), pp. 9247-9252. [10.1073/pnas.93.17.9247]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/411131
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