The pseudo EcoRI site GAATTA in the U3 region of the long terminal repeat of human immunodeficiency virus, which is flanked by a 26-base pair oligopurine tract, is readily nicked by either EcoRI or RsrI. The strand-specific nick occurs predominantly between the G and A residues and is independent of negative supercoiling. Other GAATTA sites surrounded by random (non-oligopurine) sequences are not nicked by these restriction endonucleases. However, other types and lengths of oligopurine tracts are effective in inducing the nicking in neighboring GAATTA sites. Hence, we propose that the flanking oligopurine tracts induce an altered DNA conformation on the GAATTA target site which may be similar to the transition state induced by EcoRI when binding to its canonical recognition site. Gel retardation analyses on restriction fragments containing the oligopurine-GAATTA-oligopurine sequences suggest the presence of helical axis distortions which are consistent with this interpretation.

A DNA CONFORMATIONAL ALTERATION INDUCED BY A NEIGHBORING OLIGOPURINE TRACT ON GAATTA ENABLES NICKING BY ECORI / Venditti, Sabrina Donatella Sonia; R. D., Wells. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - STAMPA. - 266:25(1991), pp. 16786-16790.

A DNA CONFORMATIONAL ALTERATION INDUCED BY A NEIGHBORING OLIGOPURINE TRACT ON GAATTA ENABLES NICKING BY ECORI

VENDITTI, Sabrina Donatella Sonia;
1991

Abstract

The pseudo EcoRI site GAATTA in the U3 region of the long terminal repeat of human immunodeficiency virus, which is flanked by a 26-base pair oligopurine tract, is readily nicked by either EcoRI or RsrI. The strand-specific nick occurs predominantly between the G and A residues and is independent of negative supercoiling. Other GAATTA sites surrounded by random (non-oligopurine) sequences are not nicked by these restriction endonucleases. However, other types and lengths of oligopurine tracts are effective in inducing the nicking in neighboring GAATTA sites. Hence, we propose that the flanking oligopurine tracts induce an altered DNA conformation on the GAATTA target site which may be similar to the transition state induced by EcoRI when binding to its canonical recognition site. Gel retardation analyses on restriction fragments containing the oligopurine-GAATTA-oligopurine sequences suggest the presence of helical axis distortions which are consistent with this interpretation.
1991
dna structure; hiv sequence; oligopurine
01 Pubblicazione su rivista::01a Articolo in rivista
A DNA CONFORMATIONAL ALTERATION INDUCED BY A NEIGHBORING OLIGOPURINE TRACT ON GAATTA ENABLES NICKING BY ECORI / Venditti, Sabrina Donatella Sonia; R. D., Wells. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - STAMPA. - 266:25(1991), pp. 16786-16790.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/409430
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