Background-Maternal smoking is an independent risk factor for sudden infant death syndrome (SIDS). Carbon monoxide (CO) is a major component of smoke. No information is available about the effect of CO and/or smoking on postnatal maturation of the heart. The aim of this study was to investigate the effect of prenatal exposure to CO on cellular electrophysiological maturation in male Wistar rats. Methods and Results-The patch-clamp technique was used to measure action potential (AP) and ionic currents (Ito and I Ca.L) from rat ventricular myocytes. During growth, AP duration measured at -20 and -50 mV (APD-20 and APD-50) decreased progressively in both groups; the process was significantly delayed in rats exposed prenatally to 150 ppm CO: At 4 weeks, APD-20 and APD -50 were 89.5±18.2 and 147.7±24.5 ms in CO (n=13) and 35.6±4.5 and 77.8±8.3 ms in control rats (Ctr; n=14; P<0.01 and P<0.05, respectively) and normalized at 8 weeks. At 4 weeks, the density of ICa.L was significantly higher (21.3±1.6 pA/pF, n=17, versus 15.9±1.6 pA/pF, n=22; P<0.05) and the density of I to significantly lower (9.6±1.5, n=22, versus 15.2±2.2 pA/pF, n=19; P<0.01) in CO than in Ctr and normalized thereafter. Conclusions-Prenatal CO exposure affects the physiological shortening of APD in neonatal rats. We speculate that a prolonged myocyte repolarization induced by prenatal exposure to smoke may establish a period of vulnerability for life-threatening arrhythmias in infancy.

Prenatal Exposure to Carbon Monoxide Affects Postnatal Cellular Electrophysiological Maturation of the Rat Heart: A Potential Substrate for Arrhythmogenesis in Infancy / L., Sartiani; E., Cerbai; G., Lonardo; P., De Paoli; M., Tattoli; R., Cagiano; M. R., Carratu'; Cuomo, Vincenzo; A., Mugelli. - In: CIRCULATION. - ISSN 0009-7322. - STAMPA. - 109:3(2004), pp. 419-423. [10.1161/01.cir.0000109497.73223.4d]

Prenatal Exposure to Carbon Monoxide Affects Postnatal Cellular Electrophysiological Maturation of the Rat Heart: A Potential Substrate for Arrhythmogenesis in Infancy

CUOMO, VINCENZO;
2004

Abstract

Background-Maternal smoking is an independent risk factor for sudden infant death syndrome (SIDS). Carbon monoxide (CO) is a major component of smoke. No information is available about the effect of CO and/or smoking on postnatal maturation of the heart. The aim of this study was to investigate the effect of prenatal exposure to CO on cellular electrophysiological maturation in male Wistar rats. Methods and Results-The patch-clamp technique was used to measure action potential (AP) and ionic currents (Ito and I Ca.L) from rat ventricular myocytes. During growth, AP duration measured at -20 and -50 mV (APD-20 and APD-50) decreased progressively in both groups; the process was significantly delayed in rats exposed prenatally to 150 ppm CO: At 4 weeks, APD-20 and APD -50 were 89.5±18.2 and 147.7±24.5 ms in CO (n=13) and 35.6±4.5 and 77.8±8.3 ms in control rats (Ctr; n=14; P<0.01 and P<0.05, respectively) and normalized at 8 weeks. At 4 weeks, the density of ICa.L was significantly higher (21.3±1.6 pA/pF, n=17, versus 15.9±1.6 pA/pF, n=22; P<0.05) and the density of I to significantly lower (9.6±1.5, n=22, versus 15.2±2.2 pA/pF, n=19; P<0.01) in CO than in Ctr and normalized thereafter. Conclusions-Prenatal CO exposure affects the physiological shortening of APD in neonatal rats. We speculate that a prolonged myocyte repolarization induced by prenatal exposure to smoke may establish a period of vulnerability for life-threatening arrhythmias in infancy.
2004
carbon monoxide; death; sudden; infant; electrophysiology; ion channels; smoking
01 Pubblicazione su rivista::01a Articolo in rivista
Prenatal Exposure to Carbon Monoxide Affects Postnatal Cellular Electrophysiological Maturation of the Rat Heart: A Potential Substrate for Arrhythmogenesis in Infancy / L., Sartiani; E., Cerbai; G., Lonardo; P., De Paoli; M., Tattoli; R., Cagiano; M. R., Carratu'; Cuomo, Vincenzo; A., Mugelli. - In: CIRCULATION. - ISSN 0009-7322. - STAMPA. - 109:3(2004), pp. 419-423. [10.1161/01.cir.0000109497.73223.4d]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/40312
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