In order to utilize virosomes or proteoliposomes for the delivery of drugs or macromolecules to specific pathologic target cells we elaborated a system to shuttle drugs to solid tissue (liver) as well as to the macrophages, a crucial cellular compartment of the immune system. Using virosomes prepared from the P3HR1 strain of Epstein-Barr virus, we demonstrated that these particles fused with human hepatocarcinoma cell line LiCA and therefore might be used as drug vectors. Furthermore, we report that proteoliposomes prepared by reconstituting in a cocktail of phosphatidylserine-phosphatidylcholine the anion transporter band 3 protein markedly increased the phagocytic activity of macrophages in culture. This could represent a new device to be used as a drug delivery system to enhance specific macrophagic functions.
ENGINEERED LIPOSOMES AND VIROSOMES FOR DELIVERY OF MACROMOLECULES / S., Grimaldi; Giuliani, Anna; Giuliani, Andrea; L., Ferroni; A., Lisi; N., Santoro; D., Pozzi. - In: RESEARCH IN VIROLOGY. - ISSN 0923-2516. - 146:4(1995), pp. 289-293. (Intervento presentato al convegno Symposium on the Prevention of Viral Hepatitis and Hepatocellular Carcinoma - Vaccines and Immunomodulants on the Threshold of the Year 2000 tenutosi a CAPRI, ITALY nel OCT 21-22, 1994) [10.1016/0923-2516(96)80574-7].
ENGINEERED LIPOSOMES AND VIROSOMES FOR DELIVERY OF MACROMOLECULES
GIULIANI, Anna;GIULIANI, Andrea;
1995
Abstract
In order to utilize virosomes or proteoliposomes for the delivery of drugs or macromolecules to specific pathologic target cells we elaborated a system to shuttle drugs to solid tissue (liver) as well as to the macrophages, a crucial cellular compartment of the immune system. Using virosomes prepared from the P3HR1 strain of Epstein-Barr virus, we demonstrated that these particles fused with human hepatocarcinoma cell line LiCA and therefore might be used as drug vectors. Furthermore, we report that proteoliposomes prepared by reconstituting in a cocktail of phosphatidylserine-phosphatidylcholine the anion transporter band 3 protein markedly increased the phagocytic activity of macrophages in culture. This could represent a new device to be used as a drug delivery system to enhance specific macrophagic functions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
