The maturation of cytolytic T lymphocytes from non-lytic precursors requires cytokines other than IL-2. Interleukin-6 is the principal cytokine that cooperates with IL-2 in the induction of CTL differentiation from murine and human thymocyte precursors. However, a cytotoxic differentiation factor role for IL-6 on mature T cells is challenged by data that indicate that IL-2 alone is sufficient for CTL generation. The aim of this study was to identify a model system in which IL-6 acted as a CDF for human peripheral T cells. Experiments that compared the cytolytic function of CTL clones cultured with and without IL-6 demonstrated that IL-6 contributes to the cytolytic activity of human CTL clones. We also demonstrate that IL-6 is endogenously produced by CTL clones in the course of their expansion with APC, lectin and IL-2. The majority of both CD4+ and CD8+ clones produced IL-6 in response to relatively high doses of IL-2. Our data suggest that IL-6 acts in an autocrine fashion to support CTL differentiation in human T-cell clones.
ROLE OF INTERLEUKIN-6 IN THE GENERATION OF HUMAN CYTOLYTIC T-CELL CLONES / Galandrini, Ricciarda; C., Cernetti; N., Albi; C., Dembech; A., Terenzi; R., Tognellini; A., Velardi. - In: JOURNAL OF IMMUNOLOGICAL RESEARCH. - ISSN 1120-3765. - STAMPA. - 3:(1991), pp. 56-61.
ROLE OF INTERLEUKIN-6 IN THE GENERATION OF HUMAN CYTOLYTIC T-CELL CLONES
GALANDRINI, Ricciarda;
1991
Abstract
The maturation of cytolytic T lymphocytes from non-lytic precursors requires cytokines other than IL-2. Interleukin-6 is the principal cytokine that cooperates with IL-2 in the induction of CTL differentiation from murine and human thymocyte precursors. However, a cytotoxic differentiation factor role for IL-6 on mature T cells is challenged by data that indicate that IL-2 alone is sufficient for CTL generation. The aim of this study was to identify a model system in which IL-6 acted as a CDF for human peripheral T cells. Experiments that compared the cytolytic function of CTL clones cultured with and without IL-6 demonstrated that IL-6 contributes to the cytolytic activity of human CTL clones. We also demonstrate that IL-6 is endogenously produced by CTL clones in the course of their expansion with APC, lectin and IL-2. The majority of both CD4+ and CD8+ clones produced IL-6 in response to relatively high doses of IL-2. Our data suggest that IL-6 acts in an autocrine fashion to support CTL differentiation in human T-cell clones.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.