High-throughput genomics requires tumor DNA matched to germline DNA, that cannot be easily obtained in the context of leukemia. Using chronic lymphocytic leukemia as a model, saliva DNA was frequently devoid of tumor DNA also during overt disease, and passed quality controls for SNP-array (77/102, 75.4%) and next generation sequencing (71/102, 69.6%). Compared to saliva, urine provides germline DNA of similar quality but in lower amounts. Saliva DNA was successfully run on SNP 6.0 arrays, and passed quality control call rate thresholds. On these bases, saliva represents a useful source of germline DNA for high-throughput genomic studies of hematologic neoplasia. (C) 2011 Elsevier Ltd. All rights reserved.
Saliva is a reliable and practical source of germline DNA for genome-wide studies in chronic lymphocytic leukemia / Silvia, Rasi; Alessio, Bruscaggin; Andrea, Rinaldi; Stefania, Cresta; Marco, Fangazio; Lorenzo De, Paoli; Sara, Monti; Ernesto, Gargiulo; Ivo, Kwee; Foa, Roberto; Francesco, Bertoni; Gianluca, Gaidano; Davide, Rossi. - In: LEUKEMIA RESEARCH. - ISSN 0145-2126. - 35:10(2011), pp. 1419-1422. [10.1016/j.leukres.2011.05.024]
Saliva is a reliable and practical source of germline DNA for genome-wide studies in chronic lymphocytic leukemia
FOA, Roberto;
2011
Abstract
High-throughput genomics requires tumor DNA matched to germline DNA, that cannot be easily obtained in the context of leukemia. Using chronic lymphocytic leukemia as a model, saliva DNA was frequently devoid of tumor DNA also during overt disease, and passed quality controls for SNP-array (77/102, 75.4%) and next generation sequencing (71/102, 69.6%). Compared to saliva, urine provides germline DNA of similar quality but in lower amounts. Saliva DNA was successfully run on SNP 6.0 arrays, and passed quality control call rate thresholds. On these bases, saliva represents a useful source of germline DNA for high-throughput genomic studies of hematologic neoplasia. (C) 2011 Elsevier Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
