The role of cyclic nucleotide-gated (CNG) channels in sensory sig- nal transduction in retinal and olfactory cells is widely recognized, but there is increasing evidence that they also play more general functions in the central nervous system as downstream e¡ectors of cyclic nucleotides. Here, we demonstrate the expression of the a-subunit of rod- and olfactory-type CNG channels (CNG1 and CNG2, respectively) in the rat medial vestibular nucleus (MVN). Nested polymerase chain reaction revealed CNG channel mRNA in the MVN, and CNG1 and CNG2 proteins were also detected by Western blotting and immunohistochemistry. Finally, electrophy- siological evidence is provided suggesting that CNG channels play a functional role in the MVN. NeuroReport 16:1939^1943 c2005 Lippincott Williams & Wilkins.
Expression of cyclic nucleotide-gated channels in the rat medial vestibular nucleus / Maria V., Podda; Marcocci, Maria Elena; B., Del Carlo; Palamara, ANNA TERESA; Gian Battista, Azzena; Claudio, Grassi. - In: NEUROREPORT. - ISSN 0959-4965. - 16:17(2005), pp. 1939-1943. [10.1097/01.wnr.0000187633.91375.c8]
Expression of cyclic nucleotide-gated channels in the rat medial vestibular nucleus
MARCOCCI, Maria Elena;PALAMARA, ANNA TERESA;
2005
Abstract
The role of cyclic nucleotide-gated (CNG) channels in sensory sig- nal transduction in retinal and olfactory cells is widely recognized, but there is increasing evidence that they also play more general functions in the central nervous system as downstream e¡ectors of cyclic nucleotides. Here, we demonstrate the expression of the a-subunit of rod- and olfactory-type CNG channels (CNG1 and CNG2, respectively) in the rat medial vestibular nucleus (MVN). Nested polymerase chain reaction revealed CNG channel mRNA in the MVN, and CNG1 and CNG2 proteins were also detected by Western blotting and immunohistochemistry. Finally, electrophy- siological evidence is provided suggesting that CNG channels play a functional role in the MVN. NeuroReport 16:1939^1943 c2005 Lippincott Williams & Wilkins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
