One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed only 1 strain with "low discrimination" whereas the VITEK2 gave 12. In any case, the two biochemical systems showed good reliability compared to SS-PCR.
Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical system and species-specific PCR / Giordano, Alessandra; Magni, Annarita; Trancassini, Maria; Varesi, Paola; Turner, R.; Mancini, Carlo. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - 64(1):(2006), pp. 135-138. [10.1016/j.mimet.2005.04.020]
Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical system and species-specific PCR
GIORDANO, Alessandra;MAGNI, Annarita;TRANCASSINI, Maria;VARESI, PAOLA;MANCINI, Carlo
2006
Abstract
One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed only 1 strain with "low discrimination" whereas the VITEK2 gave 12. In any case, the two biochemical systems showed good reliability compared to SS-PCR.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
