Objective: To study the effect of c-FLIP overexpression in testicular germ Cells. Design: A novel transgenic mouse model overexpressing the apoptotic modulator c-FLIP in the testis was generated. Setting: Animal facility and university research laboratory. Animal(s): Transgenic mice overexpressing the long isoform of c-FLIP (c-FLIP(L)) under the transcriptional control of a 400 bp long regulatory region of the Stra8 promoter. Intervention(s): Spermatozoa motility and testis histological, immunohistochemical, and Western blot analyses were carried out in transgenic and control derived specimens. Main Outcome Measure(s): Testis morphology, sperm motility, and germ cell apoptosis were assayed. Results: Stra8 promoter was found to activate the ectopic overexpression of c-FLIP(L) in round and elongated spermatids. As a consequence of such overexpression, a dramatic loss of germ cells was observed, resulting in testicular atrophy associated with reduced sperm motility. Conclusion(s): The data show that c-FLIP(L) forced expression in haploid male germ cells has detrimental effects on spermatogenesis and sperm quality and reveal a possible mechanism underlying the onset of testicular atrophy. (Fertil Steril (R) 2010;93:1407-14. (C)2010 by American Society for Reproductive Medicine.)

Testis atrophy and reduced sperm motility in transgenic mice overexpressing c-FLIP(L) / Antonangeli, Fabrizio; Petrungaro, Simonetta; Coluccia, Pier Paolo; Filippini, Antonio; Ziparo, Elio; Giampietri, Claudia. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - 93:5(2010), pp. 1407-1414. [10.1016/j.fertnstert.2009.01.122]

Testis atrophy and reduced sperm motility in transgenic mice overexpressing c-FLIP(L)

ANTONANGELI, Fabrizio;PETRUNGARO, Simonetta;COLUCCIA, Pier Paolo;FILIPPINI, Antonio;ZIPARO, Elio;GIAMPIETRI, Claudia
2010

Abstract

Objective: To study the effect of c-FLIP overexpression in testicular germ Cells. Design: A novel transgenic mouse model overexpressing the apoptotic modulator c-FLIP in the testis was generated. Setting: Animal facility and university research laboratory. Animal(s): Transgenic mice overexpressing the long isoform of c-FLIP (c-FLIP(L)) under the transcriptional control of a 400 bp long regulatory region of the Stra8 promoter. Intervention(s): Spermatozoa motility and testis histological, immunohistochemical, and Western blot analyses were carried out in transgenic and control derived specimens. Main Outcome Measure(s): Testis morphology, sperm motility, and germ cell apoptosis were assayed. Results: Stra8 promoter was found to activate the ectopic overexpression of c-FLIP(L) in round and elongated spermatids. As a consequence of such overexpression, a dramatic loss of germ cells was observed, resulting in testicular atrophy associated with reduced sperm motility. Conclusion(s): The data show that c-FLIP(L) forced expression in haploid male germ cells has detrimental effects on spermatogenesis and sperm quality and reveal a possible mechanism underlying the onset of testicular atrophy. (Fertil Steril (R) 2010;93:1407-14. (C)2010 by American Society for Reproductive Medicine.)
2010
stra8; germ cells; c-flip; apoptosis; transgenic mouse; spermatogenesis; testis atrophy
01 Pubblicazione su rivista::01a Articolo in rivista
Testis atrophy and reduced sperm motility in transgenic mice overexpressing c-FLIP(L) / Antonangeli, Fabrizio; Petrungaro, Simonetta; Coluccia, Pier Paolo; Filippini, Antonio; Ziparo, Elio; Giampietri, Claudia. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - 93:5(2010), pp. 1407-1414. [10.1016/j.fertnstert.2009.01.122]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/357860
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