A simple thin-layer chromatographic method for the separation of D- and L-dansylamino acids using a new resolving agent is described. The enantiomeric resolution of racemates was accomplished on reversed-phase plates pre-treated with a poly-L-phenylalaninamide - copper(II) complex using water- acetonitrile mixtures as eluent under isocratic conditions. The polymeric ligand was easily prepared by reaction of the optically active amide with ethylene glycol diglycidyl ether at room temperature. The method is rapid and sensitive, and was successfully employed to resolve various dansylamino acids.
SEPARATION OF DANSYLAMINO ACID ENANTIOMERS BY THIN-LAYER CHROMATOGRAPHY / Massimo, Sinibaldi; Messina, Antonella; Girelli, Anna Maria. - In: ANALYST. - ISSN 0003-2654. - STAMPA. - 113:8(1988), pp. 1245-1247. [10.1039/an9881301245]
SEPARATION OF DANSYLAMINO ACID ENANTIOMERS BY THIN-LAYER CHROMATOGRAPHY
MESSINA, Antonella;GIRELLI, Anna Maria
1988
Abstract
A simple thin-layer chromatographic method for the separation of D- and L-dansylamino acids using a new resolving agent is described. The enantiomeric resolution of racemates was accomplished on reversed-phase plates pre-treated with a poly-L-phenylalaninamide - copper(II) complex using water- acetonitrile mixtures as eluent under isocratic conditions. The polymeric ligand was easily prepared by reaction of the optically active amide with ethylene glycol diglycidyl ether at room temperature. The method is rapid and sensitive, and was successfully employed to resolve various dansylamino acids.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.