We recently reported that human dendritic cells release the leaderless secretary protein Interleukin-1 beta (IL-1 beta) following specific Interaction with alloreactive T lymphocytes. To clarity the molecular mechanism underlying this secretion, this study investigated the intracellular trafficking of IL-1 beta in dendritic cells and the signal(s) regulating its release. Results show that a fraction of the intracellular IL-1 beta precursor colocalizes with the hydrolase cathepsin D in endolysosomes of dendritic cells; secretion of both proteins Is elicited by stimuli that induce intracellular calcium increases. Alloreactive CD8(+) T lymphocytes generate a Ca++ influx in dendritic cells followed by enrichment in endolysosomes containing IL-1 beta and cathepsin D beneath the membrane In contact with T cells. These events result in polarized exocytosis of secretary lysosomes, mediated by microtubules, with release of IL-1 beta and cathepsin D toward the interacting CD8(+) T cell. (C) 2001 by The American Society of Hematology.

CD8+ T lymphocytes induce polarized exocytosis of secretory lysosomes by dendritic cells with release of interleukin-1-Beta and cathepsin D / S., Gardella; C., Andrei; Lotti, Lavinia Vittoria; A., Poggi; Torrisi, Maria Rosaria; M. R., Zocchi; A., Rubartelli. - In: BLOOD. - ISSN 0006-4971. - STAMPA. - 98:(2001), pp. 2152-2159. [10.1182/blood.V98.7.2152]

CD8+ T lymphocytes induce polarized exocytosis of secretory lysosomes by dendritic cells with release of interleukin-1-Beta and cathepsin D

LOTTI, Lavinia Vittoria;TORRISI, Maria Rosaria;
2001

Abstract

We recently reported that human dendritic cells release the leaderless secretary protein Interleukin-1 beta (IL-1 beta) following specific Interaction with alloreactive T lymphocytes. To clarity the molecular mechanism underlying this secretion, this study investigated the intracellular trafficking of IL-1 beta in dendritic cells and the signal(s) regulating its release. Results show that a fraction of the intracellular IL-1 beta precursor colocalizes with the hydrolase cathepsin D in endolysosomes of dendritic cells; secretion of both proteins Is elicited by stimuli that induce intracellular calcium increases. Alloreactive CD8(+) T lymphocytes generate a Ca++ influx in dendritic cells followed by enrichment in endolysosomes containing IL-1 beta and cathepsin D beneath the membrane In contact with T cells. These events result in polarized exocytosis of secretary lysosomes, mediated by microtubules, with release of IL-1 beta and cathepsin D toward the interacting CD8(+) T cell. (C) 2001 by The American Society of Hematology.
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CD8+ T lymphocytes induce polarized exocytosis of secretory lysosomes by dendritic cells with release of interleukin-1-Beta and cathepsin D / S., Gardella; C., Andrei; Lotti, Lavinia Vittoria; A., Poggi; Torrisi, Maria Rosaria; M. R., Zocchi; A., Rubartelli. - In: BLOOD. - ISSN 0006-4971. - STAMPA. - 98:(2001), pp. 2152-2159. [10.1182/blood.V98.7.2152]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/27817
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