Experiments carried out with hepatocytes entrapped in alginate gel particles with a cell density of about 107cells ml-1showed an oxygen consumption rate of about 2.2 x 10-16mol cell-1s-1. Under these conditions, no inner anoxic core is present in 1.8 mm diameter beads. From these data, the maximum bead size consistent with noncritical O2concentration at the bead center has been evaluated for different cell densities and O2concentrations in the medium.
Experiments carried out with hepatocytes entrapped in alginate gel particles with a cell density of about 107 cells ml-1 showed an oxygen consumption rate of about 2.2 x 10-16 mol cell-1 s-1. Under these conditions, no inner anoxic core is present in 1.8 mm diameter beads. From these data, the maximum bead size consistent with noncritical O2 concentration at the bead center has been evaluated for different cell densities and O2 concentrations in the medium.
Transport and consumption rate of O2 in alginate gel beads entrapping hepatocytes / Annesini, Maria Cristina; Castelli, Giuseppe; Conti, Filippo; Conti De Virgiliis, Laura; Marrelli, Luigi; Miccheli, Alfredo; Satori, Elisabetta. - In: BIOTECHNOLOGY LETTERS. - ISSN 0141-5492. - STAMPA. - 22:10(2000), pp. 865-870. [10.1023/A:1005623732252]
Transport and consumption rate of O2 in alginate gel beads entrapping hepatocytes
ANNESINI, Maria Cristina;CONTI, Filippo;MARRELLI, Luigi;MICCHELI, Alfredo;
2000
Abstract
Experiments carried out with hepatocytes entrapped in alginate gel particles with a cell density of about 107cells ml-1showed an oxygen consumption rate of about 2.2 x 10-16mol cell-1s-1. Under these conditions, no inner anoxic core is present in 1.8 mm diameter beads. From these data, the maximum bead size consistent with noncritical O2concentration at the bead center has been evaluated for different cell densities and O2concentrations in the medium.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
