In vitro cell cultures have been widely used as a means of evaluating cytotoxicity of root canal filling materials. Following ANSI/ADA spec. no. 41, the aim of the present study was to investigate the biological compatibility of a new sealer (FibreFill) and compare it with some commercially available endodontic sealers (Bioseal and Acroseal). Mouse 3T3 fibroblasts were seeded and cultured and subsequently extracts of the cements were added. After 24 hours incubation, the cellular vitality of fibroblasts was evaluated by the neutral red uptake test (NRU), which measures the membrane permeability. Data were collected and statistically analysed. Results showed that all tested materials exhibited mild cytotoxic effects, which are compatible with normal clinical use, and no statistically significant difference was noted between FibreFill and the other tested materials. Therefore, selection amongst these sealers should be based on other factors.

Cytotoxicity of a new endodontic filling material / Gambarini, Gianluca; M., Andreasi Bassi; G., Bolognini; Testarelli, Luca; G., Nocca; L., Ceccarelli; R., Scatena; A., Lupi; M., Castagnola. - In: AUSTRALIAN ENDODONTIC JOURNAL. - ISSN 1329-1947. - 29:1(2003), pp. 17-19. [10.1111/j.1747-4477.2003.tb00489.x]

Cytotoxicity of a new endodontic filling material

GAMBARINI, Gianluca;TESTARELLI, Luca;
2003

Abstract

In vitro cell cultures have been widely used as a means of evaluating cytotoxicity of root canal filling materials. Following ANSI/ADA spec. no. 41, the aim of the present study was to investigate the biological compatibility of a new sealer (FibreFill) and compare it with some commercially available endodontic sealers (Bioseal and Acroseal). Mouse 3T3 fibroblasts were seeded and cultured and subsequently extracts of the cements were added. After 24 hours incubation, the cellular vitality of fibroblasts was evaluated by the neutral red uptake test (NRU), which measures the membrane permeability. Data were collected and statistically analysed. Results showed that all tested materials exhibited mild cytotoxic effects, which are compatible with normal clinical use, and no statistically significant difference was noted between FibreFill and the other tested materials. Therefore, selection amongst these sealers should be based on other factors.
2003
01 Pubblicazione su rivista::01a Articolo in rivista
Cytotoxicity of a new endodontic filling material / Gambarini, Gianluca; M., Andreasi Bassi; G., Bolognini; Testarelli, Luca; G., Nocca; L., Ceccarelli; R., Scatena; A., Lupi; M., Castagnola. - In: AUSTRALIAN ENDODONTIC JOURNAL. - ISSN 1329-1947. - 29:1(2003), pp. 17-19. [10.1111/j.1747-4477.2003.tb00489.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/250170
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