Common variable immunodeficiency (CVID) is a heterogeneous syndrome characterized by repeated infections and hypogammaglobulinaemia. Additionally, T-cell abnormalities including lymphopenia, decreased proliferation to mitogens and antigens, and the reduced production and expression of cytokines, have also been observed. In this study we have investigated the expression of naive, memory and activation markers in T-cell subpopulations in 17 CVID patients in comparison to age-matched normal controls. The numbers of CD4(+) T cells, including CD45RA(+)CD62L(+) and, to a lesser extent, CD45RA(-)CD62L(+)/RA(+)CD62L(-) were significantly reduced in patients, whereas CD8(+) T cells were within normal range. In contrast, HLA-DR+ cells were increased both in CD4(+) and CD8+ T cells. To assess the thymic output, we analysed the presence of T-cell receptor excision circles (TRECs) in CD4(+) and CD8(+) T cells by quantitative PCR. TRECs were decreased significantly in patients and the rate of TREC loss was higher with increasing age. TRECs correlated with naive CD4(+) T cells, whereas there was an inverse relationship between TRECs and CD8(+)HLA(-)DR(+) and CD8(+)CD45RA(-)CD62L(+)/RA(+)CD62L(-) T cells. Our results suggest the presence of a defect in the naive T cell compartment with origin at the thymic level in CVID, and indicate that TREC may be a useful marker to monitor thymic function in this primary immunodeficiency.

Assessment of thymic output in common variable immunodeficiency patients by evaluation of T cell receptor excision circles / V., Guazzi; Aiuti, Fernando; Mezzaroma, Ivano; F., Mazzetta; G., Andolfi; A., Mortellaro; M., Pierdominici; R., Fantini; M., Marziali; A., Aiuti. - In: CLINICAL AND EXPERIMENTAL IMMUNOLOGY. - ISSN 0009-9104. - STAMPA. - 129:2(2002), pp. 346-353. [10.1046/j.1365-2249.2002.01893.x]

Assessment of thymic output in common variable immunodeficiency patients by evaluation of T cell receptor excision circles

AIUTI, Fernando;MEZZAROMA, Ivano;
2002

Abstract

Common variable immunodeficiency (CVID) is a heterogeneous syndrome characterized by repeated infections and hypogammaglobulinaemia. Additionally, T-cell abnormalities including lymphopenia, decreased proliferation to mitogens and antigens, and the reduced production and expression of cytokines, have also been observed. In this study we have investigated the expression of naive, memory and activation markers in T-cell subpopulations in 17 CVID patients in comparison to age-matched normal controls. The numbers of CD4(+) T cells, including CD45RA(+)CD62L(+) and, to a lesser extent, CD45RA(-)CD62L(+)/RA(+)CD62L(-) were significantly reduced in patients, whereas CD8(+) T cells were within normal range. In contrast, HLA-DR+ cells were increased both in CD4(+) and CD8+ T cells. To assess the thymic output, we analysed the presence of T-cell receptor excision circles (TRECs) in CD4(+) and CD8(+) T cells by quantitative PCR. TRECs were decreased significantly in patients and the rate of TREC loss was higher with increasing age. TRECs correlated with naive CD4(+) T cells, whereas there was an inverse relationship between TRECs and CD8(+)HLA(-)DR(+) and CD8(+)CD45RA(-)CD62L(+)/RA(+)CD62L(-) T cells. Our results suggest the presence of a defect in the naive T cell compartment with origin at the thymic level in CVID, and indicate that TREC may be a useful marker to monitor thymic function in this primary immunodeficiency.
2002
diseases; gene; immunodeficiency; lymphocytes; rearrangement; t; t lymphocytes; thymus
01 Pubblicazione su rivista::01a Articolo in rivista
Assessment of thymic output in common variable immunodeficiency patients by evaluation of T cell receptor excision circles / V., Guazzi; Aiuti, Fernando; Mezzaroma, Ivano; F., Mazzetta; G., Andolfi; A., Mortellaro; M., Pierdominici; R., Fantini; M., Marziali; A., Aiuti. - In: CLINICAL AND EXPERIMENTAL IMMUNOLOGY. - ISSN 0009-9104. - STAMPA. - 129:2(2002), pp. 346-353. [10.1046/j.1365-2249.2002.01893.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/248941
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