High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99mTc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability
Efficient one-step direct labelling of recombinant antibodies with technetium-99m / Liberatore, Mauro; D., Neri; G., Neri; A., Pini; A. P., Iurilli; F., Ponzo; G., Spampinato; F., Padula; Pala, Alessandro; A. C., Colella. - In: EUROPEAN JOURNAL OF NUCLEAR MEDICINE. - ISSN 0340-6997. - STAMPA. - 22:11(1995), pp. 1326-1329. [10.1007/bf00801622]
Efficient one-step direct labelling of recombinant antibodies with technetium-99m
LIBERATORE, Mauro;PALA, Alessandro;
1995
Abstract
High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99mTc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stabilityI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
