Fractionation of internal matrix proteins from chicken liver nuclei led to the isolation of a 32 kDa protein which was identified by partial aminoacid sequence and immunological analysis as annexin V, an unreported nuclear matrix component. Our results showed that this protein is preferentially associated with the internal nuclear matrix fraction, since this is the only nuclear fraction where the protein can be immunodetected. Immunostaining on cultured cells also revealed a nuclear distribution with the exclusion of the nucleolar compartment and an association with cytosolic filamentous structures most likely corresponding to the cytoskeleton. Moreover, immunostaining on extracted cells to reveal the nuclear matrix showed a network-like distribution. Since annexin V has been reported as an inhibitor of protein kinase C, its nuclear localization in association with the internal matrix, which plays an important role in several nuclear processes, indicates its involvement in the regulation of signal transduction. (C) 1996 Academic Press, Inc.

Nuclear matrix localization of annexin V in chicken liver / Altieri, Fabio; Maras, Bruno; Turano, Carlo. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 225:2(1996), pp. 448-454. [10.1006/bbrc.1996.1193]

Nuclear matrix localization of annexin V in chicken liver

ALTIERI, Fabio;MARAS, Bruno;TURANO, Carlo
1996

Abstract

Fractionation of internal matrix proteins from chicken liver nuclei led to the isolation of a 32 kDa protein which was identified by partial aminoacid sequence and immunological analysis as annexin V, an unreported nuclear matrix component. Our results showed that this protein is preferentially associated with the internal nuclear matrix fraction, since this is the only nuclear fraction where the protein can be immunodetected. Immunostaining on cultured cells also revealed a nuclear distribution with the exclusion of the nucleolar compartment and an association with cytosolic filamentous structures most likely corresponding to the cytoskeleton. Moreover, immunostaining on extracted cells to reveal the nuclear matrix showed a network-like distribution. Since annexin V has been reported as an inhibitor of protein kinase C, its nuclear localization in association with the internal matrix, which plays an important role in several nuclear processes, indicates its involvement in the regulation of signal transduction. (C) 1996 Academic Press, Inc.
1996
01 Pubblicazione su rivista::01a Articolo in rivista
Nuclear matrix localization of annexin V in chicken liver / Altieri, Fabio; Maras, Bruno; Turano, Carlo. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 225:2(1996), pp. 448-454. [10.1006/bbrc.1996.1193]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/247231
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