4-Hydroxynonenal (HNE), a major lipid peroxidation product, effectively inhibits the superoxide radical formation by NADPH oxidase of phorbol myristate acetate (PMA)--stimulated human PMNL. The I50 value for the inhibition of NADPH oxidase-mediated superoxide radical formation by 4-hydroxynonenal was found to be 19 microM. The HNE inhibition involves the reaction with both -SH and -NH2 groups. Superoxide formation as final result of the NADPH oxidase cascade was almost completely restored by addition of dithiothreitol. In presence of hydroxylamine only a minor restoration of superoxide radical formation was found. A combination of dithiothreitol and hydroxylamine yielded the greatest recovery. Two other aldehydes with the same chain length as HNE but different binding to lysine, histidine and cysteine residues, trans-2,3-nonenal and nonanal, gave I50 values for the inhibition of NADPH oxidase-mediated superoxide formation rate of 110 microM or > 300 microM, respectively.

Inhibition of NADPH oxidase-mediated superoxide radical formation in PMA-stimulated human neutrophils by 4-hydroxynonenal-binding to -SH and NH2 groups / Siems, W. G.; Capuozzo, Elisabetta; Verginelli, D.; Salerno, Costantino; Crifo', Carlo; Grune, T.. - In: FREE RADICAL RESEARCH. - ISSN 1071-5762. - STAMPA. - 27:(1997), pp. 353-358. [10.3109/10715769709065774]

Inhibition of NADPH oxidase-mediated superoxide radical formation in PMA-stimulated human neutrophils by 4-hydroxynonenal-binding to -SH and NH2 groups.

CAPUOZZO, Elisabetta;SALERNO, Costantino;CRIFO', Carlo;
1997

Abstract

4-Hydroxynonenal (HNE), a major lipid peroxidation product, effectively inhibits the superoxide radical formation by NADPH oxidase of phorbol myristate acetate (PMA)--stimulated human PMNL. The I50 value for the inhibition of NADPH oxidase-mediated superoxide radical formation by 4-hydroxynonenal was found to be 19 microM. The HNE inhibition involves the reaction with both -SH and -NH2 groups. Superoxide formation as final result of the NADPH oxidase cascade was almost completely restored by addition of dithiothreitol. In presence of hydroxylamine only a minor restoration of superoxide radical formation was found. A combination of dithiothreitol and hydroxylamine yielded the greatest recovery. Two other aldehydes with the same chain length as HNE but different binding to lysine, histidine and cysteine residues, trans-2,3-nonenal and nonanal, gave I50 values for the inhibition of NADPH oxidase-mediated superoxide formation rate of 110 microM or > 300 microM, respectively.
1997
Superoxide radical; Neutrophil; 4-hydroxynonenal
01 Pubblicazione su rivista::01a Articolo in rivista
Inhibition of NADPH oxidase-mediated superoxide radical formation in PMA-stimulated human neutrophils by 4-hydroxynonenal-binding to -SH and NH2 groups / Siems, W. G.; Capuozzo, Elisabetta; Verginelli, D.; Salerno, Costantino; Crifo', Carlo; Grune, T.. - In: FREE RADICAL RESEARCH. - ISSN 1071-5762. - STAMPA. - 27:(1997), pp. 353-358. [10.3109/10715769709065774]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/246184
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