Abstract: The copper (II) complex of amikacin in water solution at pH 5.5 was investigated by C-13-NMR. The temperature dependence of spin-lattice relaxation rates was measured and fast exchange conditions were shown to apply. The motional correlation time of the complex was approximated by the pseudo-isotropic rotational correlation time of free amikacin in water solution (tau(c) = 0.17 ns at 300 K). Formation of a pseudo-tetrahedral 1:1 complex was demonstrated by relaxation rates analysis and also by UV-Vis spectrophotometry. Two amino nitrogens of amikacin, together with the amide nitrogen and the hydroxyl in the hydroxyl-aminopropyl carbonyl side chain, were assigned as the copper-binding sites and a model of the complex was built by using copper-carbon distances obtained by NMR analysis as input parameters.
DELINEATION OF CONFORMATIONAL AND STRUCTURAL FEATURES OF THE AMIKACIN-CU(II) COMPLEX IN WATER SOLUTION BY 13C-NMR SPECTROSCOPY / E., Gaggelli; N., Gaggelli; A., Maccotta; G., Valensin; D., Marini; DI COCCO, Maria Enrica; Manetti, Cesare; Delfini, Maurizio. - In: SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY. - ISSN 1386-1425. - STAMPA. - 55:1(1999), pp. 205-210. [10.1016/S1386-1425(98)00231-5]
DELINEATION OF CONFORMATIONAL AND STRUCTURAL FEATURES OF THE AMIKACIN-CU(II) COMPLEX IN WATER SOLUTION BY 13C-NMR SPECTROSCOPY
DI COCCO, Maria Enrica;MANETTI, Cesare;DELFINI, Maurizio
1999
Abstract
Abstract: The copper (II) complex of amikacin in water solution at pH 5.5 was investigated by C-13-NMR. The temperature dependence of spin-lattice relaxation rates was measured and fast exchange conditions were shown to apply. The motional correlation time of the complex was approximated by the pseudo-isotropic rotational correlation time of free amikacin in water solution (tau(c) = 0.17 ns at 300 K). Formation of a pseudo-tetrahedral 1:1 complex was demonstrated by relaxation rates analysis and also by UV-Vis spectrophotometry. Two amino nitrogens of amikacin, together with the amide nitrogen and the hydroxyl in the hydroxyl-aminopropyl carbonyl side chain, were assigned as the copper-binding sites and a model of the complex was built by using copper-carbon distances obtained by NMR analysis as input parameters.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.