This work presents new amperometric bienzymatic bioelectrodes for the determination of organophosphorus and carbamic acid type pesticides. Two different kinds of bienzymatic bioelectrodes are presented: a classical bienzymatic electrode, obtained by physicochemical immobilization of purified acid phosphatase (AP) and glucose oxidase (GOD) on the tip of an amperometric H2O2 electrode; and a hybrid biosensor, in which AP has been employed in the form of a thin layer of potato (Solanum tuberosum) tissue, endowed with a high content of enzyme activity. Both the biosensors can selectively detect glucose-6-phosphate (G6P), in the 5.0 × 10−5 −1.2 × 10−3M concentration range. Pesticides are detected, thanks to their high inhibition power towards AP, evaluated by adding the sample stepwise to a buffered solution of G6P, and recording the corresponding current change. The detection limit is therefore a function of the type of pesticide, but it can be as low as 1 μg 1−1 in the case of organophosphorus compounds. The detection limit is generally higher for carbamates, as a consequence of their weaker inhibition power towards acid phosphatase. Both bioelectrodes presented comparable values of the main physicochemical and analytical parameters evaluated for assessing their overall performance; nonetheless the plant tissue based bioelectrode exhibited a longer shelf life and a better reliability of the amperometric results.

Acid Phophatase/Glucose Oxidase Based Biosensors for the Determination of Pesticides / Mazzei, Franco; Botre', Francesco; Botre', Claudio. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 336:1/3(1996), pp. 67-71. [10.1016/S0003-2670(96)00378-9]

Acid Phophatase/Glucose Oxidase Based Biosensors for the Determination of Pesticides.

MAZZEI, Franco;BOTRE', Francesco;BOTRE', Claudio
1996

Abstract

This work presents new amperometric bienzymatic bioelectrodes for the determination of organophosphorus and carbamic acid type pesticides. Two different kinds of bienzymatic bioelectrodes are presented: a classical bienzymatic electrode, obtained by physicochemical immobilization of purified acid phosphatase (AP) and glucose oxidase (GOD) on the tip of an amperometric H2O2 electrode; and a hybrid biosensor, in which AP has been employed in the form of a thin layer of potato (Solanum tuberosum) tissue, endowed with a high content of enzyme activity. Both the biosensors can selectively detect glucose-6-phosphate (G6P), in the 5.0 × 10−5 −1.2 × 10−3M concentration range. Pesticides are detected, thanks to their high inhibition power towards AP, evaluated by adding the sample stepwise to a buffered solution of G6P, and recording the corresponding current change. The detection limit is therefore a function of the type of pesticide, but it can be as low as 1 μg 1−1 in the case of organophosphorus compounds. The detection limit is generally higher for carbamates, as a consequence of their weaker inhibition power towards acid phosphatase. Both bioelectrodes presented comparable values of the main physicochemical and analytical parameters evaluated for assessing their overall performance; nonetheless the plant tissue based bioelectrode exhibited a longer shelf life and a better reliability of the amperometric results.
1996
Glucose-6-phosphate; Biosensors; Enzyme inhibition; Acid phosphatase; Glucose oxidase; Pesticides
01 Pubblicazione su rivista::01a Articolo in rivista
Acid Phophatase/Glucose Oxidase Based Biosensors for the Determination of Pesticides / Mazzei, Franco; Botre', Francesco; Botre', Claudio. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 336:1/3(1996), pp. 67-71. [10.1016/S0003-2670(96)00378-9]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/245028
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 58
  • ???jsp.display-item.citation.isi??? 51
social impact