Tumor necrosis factor-alpha (TNF-alpha) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-alpha increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-alpha receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-alpha effect are discussed.
TNF-ALPHA INCREASES THE FREQUENCY OF SPONTANEOUS MINIATURE SYNAPTIC CURRENTS IN CULTURED RAT HIPPOCAMPAL-NEURONS / Grassi, Francesca; Mileo, Am; Monaco, Lucia; Punturieri, A; Santoni, Angela; Eusebi, Fabrizio. - In: BRAIN RESEARCH. - ISSN 0006-8993. - STAMPA. - 659:(1994), pp. 226-230. [10.1016/0006-8993(94)90883-4]
TNF-ALPHA INCREASES THE FREQUENCY OF SPONTANEOUS MINIATURE SYNAPTIC CURRENTS IN CULTURED RAT HIPPOCAMPAL-NEURONS
GRASSI, Francesca;MONACO, Lucia;SANTONI, Angela;EUSEBI, Fabrizio
1994
Abstract
Tumor necrosis factor-alpha (TNF-alpha) is a cytokine secreted by activated astrocytes and is known to alter evoked synaptic activity in slices of adult rat hippocampus. In this paper we show that TNF-alpha increases the frequency of spontaneous miniature synaptic currents in cultured hippocampal neurons, acting at nanomolar concentrations. In addition, we show that the mRNA for the 55 kDa TNF-alpha receptor (TNF-R1) is detected in embryonic rat hippocampal cultures, as well as in acutely dissected embryonic and adult rat hippocampi. Possible transduction pathways mediating the TNF-alpha effect are discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
