In early mouse embryos, the major inducible heat shock gene, hsp68, is spontaneously and transiently activated at the two-cell stage and becomes heat-inducible around blastocyst stage. We have probed mouse embryo's ability to activate the promoter of this gene during preimplantation development by expression analysis of DNA constructs containing a reporter lacZ gene driven by hsp68 (hsp70Al) 5'-regulatory sequences of various length: (i) a full-length promoter (construct phsplacZ); (ii) a heat shock element (HSE)-deleted promoter (p Delta 1hsplacZ); and (iii) a minimal, proximal promoter (p Delta 2hsplacZ). When analyzed in transfected L-cells, phsplacZ was heat-inducible, while neither p Delta 1hsplacZ nor p Delta 2hsplacZ was. Developmental activity of the full-length construct was first analyzed after genome integration in transgenic embryos and found to follow endogenous hsp68 expression in terms of spontaneous activation at the 2-cell stage, down-regulation at the 4-cell stage, and acquisition of heat inducibility at the 16/32-cell stage. In transient expression experiments, injected phsplacZ, p Delta 1hsplacZ, and p Delta 2hsplacZ were expressed at similar levels by 2-cell embryos, independently of construct topology and injection stage. At the 4-cell stage, however, phsplacZ and and p Delta 1hsplacZ were expressed at similar levels, while p Delta 2hsplacZ was inactive. Only phsplacZ became heat-inducible in late morulas. We conclude that in early mouse embryos, developmental activity of episomic hsp68 promoter depends on proximal sequences at the 2-cell stage and on putative enhancer sequences at the 4-cell stage, while HSEs appear dispensable during early cleavage. (C) 1995 Academic Press, Inc.

STAGE-SPECIFIC REGULATION OF MURINE HSP68 GENE PROMOTER IN PREIMPLANTATION MOUSE EMBRYOS / Bevilacqua, Arturo; Leann H., Kinnunen; Stefania, Bevilacqua; Mangia, Franco. - In: DEVELOPMENTAL BIOLOGY. - ISSN 0012-1606. - STAMPA. - 170:2(1995), pp. 467-478. [10.1006/dbio.1995.1230]

STAGE-SPECIFIC REGULATION OF MURINE HSP68 GENE PROMOTER IN PREIMPLANTATION MOUSE EMBRYOS

BEVILACQUA, Arturo;MANGIA, Franco
1995

Abstract

In early mouse embryos, the major inducible heat shock gene, hsp68, is spontaneously and transiently activated at the two-cell stage and becomes heat-inducible around blastocyst stage. We have probed mouse embryo's ability to activate the promoter of this gene during preimplantation development by expression analysis of DNA constructs containing a reporter lacZ gene driven by hsp68 (hsp70Al) 5'-regulatory sequences of various length: (i) a full-length promoter (construct phsplacZ); (ii) a heat shock element (HSE)-deleted promoter (p Delta 1hsplacZ); and (iii) a minimal, proximal promoter (p Delta 2hsplacZ). When analyzed in transfected L-cells, phsplacZ was heat-inducible, while neither p Delta 1hsplacZ nor p Delta 2hsplacZ was. Developmental activity of the full-length construct was first analyzed after genome integration in transgenic embryos and found to follow endogenous hsp68 expression in terms of spontaneous activation at the 2-cell stage, down-regulation at the 4-cell stage, and acquisition of heat inducibility at the 16/32-cell stage. In transient expression experiments, injected phsplacZ, p Delta 1hsplacZ, and p Delta 2hsplacZ were expressed at similar levels by 2-cell embryos, independently of construct topology and injection stage. At the 4-cell stage, however, phsplacZ and and p Delta 1hsplacZ were expressed at similar levels, while p Delta 2hsplacZ was inactive. Only phsplacZ became heat-inducible in late morulas. We conclude that in early mouse embryos, developmental activity of episomic hsp68 promoter depends on proximal sequences at the 2-cell stage and on putative enhancer sequences at the 4-cell stage, while HSEs appear dispensable during early cleavage. (C) 1995 Academic Press, Inc.
1995
01 Pubblicazione su rivista::01a Articolo in rivista
STAGE-SPECIFIC REGULATION OF MURINE HSP68 GENE PROMOTER IN PREIMPLANTATION MOUSE EMBRYOS / Bevilacqua, Arturo; Leann H., Kinnunen; Stefania, Bevilacqua; Mangia, Franco. - In: DEVELOPMENTAL BIOLOGY. - ISSN 0012-1606. - STAMPA. - 170:2(1995), pp. 467-478. [10.1006/dbio.1995.1230]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/243746
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