From skin secretions of Bombina Íariegata and Bombina bombina, we isolated a small protein termed Bv8. The sequence of its 77 amino acids was established by peptide analysis and by cDNA cloning of the Bv8 precursor. Bv8 stimulates the contraction of the guinea-pig ileum at nanomolar concentrations. The contraction is not inhibited by a variety of antagonists. Injection of a few micrograms of Bv8 into the brain of rats elicits, as assessed by the tail-flick test and paw pressure threshold, a marked hyperalgesia which lasts for about 1 h. Bv8 is related to protein A, a component of the venom of the black mamba. After i.c.v. injection, protein A is even more active than Bv8 in inducing hyperalgesia.

Bv8, a small protein from frog skin and its homologue from snake venom induce hyperalgesia in rats / Christa, Mollay; Christian, Wechselberger; Mignogna, Giuseppina; Negri, Lucia; Melchiorri, Pietro; Barra, Donatella; Günther, Kreil. - In: EUROPEAN JOURNAL OF PHARMACOLOGY. - ISSN 0014-2999. - STAMPA. - 374:2(1999), pp. 189-196. [10.1016/s0014-2999(99)00229-0]

Bv8, a small protein from frog skin and its homologue from snake venom induce hyperalgesia in rats

MIGNOGNA, Giuseppina;NEGRI, Lucia;MELCHIORRI, Pietro;BARRA, Donatella;
1999

Abstract

From skin secretions of Bombina Íariegata and Bombina bombina, we isolated a small protein termed Bv8. The sequence of its 77 amino acids was established by peptide analysis and by cDNA cloning of the Bv8 precursor. Bv8 stimulates the contraction of the guinea-pig ileum at nanomolar concentrations. The contraction is not inhibited by a variety of antagonists. Injection of a few micrograms of Bv8 into the brain of rats elicits, as assessed by the tail-flick test and paw pressure threshold, a marked hyperalgesia which lasts for about 1 h. Bv8 is related to protein A, a component of the venom of the black mamba. After i.c.v. injection, protein A is even more active than Bv8 in inducing hyperalgesia.
1999
bv8 (bombina variegata protein 8 kda); guinea pig; hyperalgesia; rat; ileum
01 Pubblicazione su rivista::01a Articolo in rivista
Bv8, a small protein from frog skin and its homologue from snake venom induce hyperalgesia in rats / Christa, Mollay; Christian, Wechselberger; Mignogna, Giuseppina; Negri, Lucia; Melchiorri, Pietro; Barra, Donatella; Günther, Kreil. - In: EUROPEAN JOURNAL OF PHARMACOLOGY. - ISSN 0014-2999. - STAMPA. - 374:2(1999), pp. 189-196. [10.1016/s0014-2999(99)00229-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/242875
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