A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6, The effects of the substances were separately tested on both penetration and intracellular multiplication of L. pneumophila, Only cytochalasin A and 2-deoxy-D-glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose-response effect. The action of 2dG was counteracted by 50 mM glucose. Experiments carried out with cytochalasin D and a rhodamine-phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella; compounds acting on microtubules had no effect.
Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors / Goldoni, Paola; L., Cattani; S., Carrara; M., Castellani Pastoris; Sinibaldi, Laura; Orsi, Nicola. - In: MICROBIOLOGY AND IMMUNOLOGY. - ISSN 0385-5600. - 42:4(1998), pp. 271-279.
Multiplication of Legionella pneumophila in HeLa cells in the presence of cytoskeleton and metabolic inhibitors
GOLDONI, Paola;SINIBALDI, Laura;ORSI, Nicola
1998
Abstract
A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6, The effects of the substances were separately tested on both penetration and intracellular multiplication of L. pneumophila, Only cytochalasin A and 2-deoxy-D-glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose-response effect. The action of 2dG was counteracted by 50 mM glucose. Experiments carried out with cytochalasin D and a rhodamine-phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella; compounds acting on microtubules had no effect.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.