To study how subunit composition affects the functional properties of neuronal nicotinic acetylcholine receptors (nAChRs), we examined the behaviour of acetylcholine (ACh)-induced single-channel currents in human BOSC 23 cells transiently transfected with various subunit cDNA combinations. For all nAChRs examined (chick and rat alpha 3 beta 4, chick alpha 3 beta 2, alpha 4 beta 2, alpha 7 and alpha 8), expression levels were high enough to allow measurements of acetylcholine-evoked whole-cell currents and nicotine-elicited Ca2+ transients as well as the functional characterization of nAChR channels. Unitary acetylcholine-evoked events of alpha 8 nAChR had a slope conductance of 23 pS, whereas two conductance classes (19-23 and 32-45 pS) were identified for all other nAChR channels. The mean channel open times were significantly longer for homomeric alpha 7 and alpha 8 nAChRs (6-7 ms) than for heteromeric nAChRs (1-3 ms), with the exception of alpha 3 beta 4 nAChRs (8.4 ms for rat, 7 ms for chick). At least two species of heterologously expressed nAChRs (alpha 3 beta 4 and alpha 3 beta 2) exhibited single-channel characteristics similar to those reported for native receptors. The variety of nAChR channel conductance and kinetic properties encountered in human cells transfected with nAChR subunits contributes to the functional diversity of nAChRs in nerve cells.

Functional properties of neuronal nicotinic acetylcholine receptor-channels expressed in transfected human cells / Ragozzino, Davide Antonio; Fucile, Sergio; Giovannelli, A.; Grassi, Francesca; Mileo, A. M.; Ballivet, M.; Alemà, S.; Eusebi, Fabrizio. - In: EUROPEAN JOURNAL OF NEUROSCIENCE. - ISSN 0953-816X. - STAMPA. - 9:(1997), pp. 480-488. [10.1111/j.1460-9568.1997.tb01625.x]

Functional properties of neuronal nicotinic acetylcholine receptor-channels expressed in transfected human cells

RAGOZZINO, Davide Antonio;FUCILE, Sergio;GRASSI, Francesca;EUSEBI, Fabrizio
1997

Abstract

To study how subunit composition affects the functional properties of neuronal nicotinic acetylcholine receptors (nAChRs), we examined the behaviour of acetylcholine (ACh)-induced single-channel currents in human BOSC 23 cells transiently transfected with various subunit cDNA combinations. For all nAChRs examined (chick and rat alpha 3 beta 4, chick alpha 3 beta 2, alpha 4 beta 2, alpha 7 and alpha 8), expression levels were high enough to allow measurements of acetylcholine-evoked whole-cell currents and nicotine-elicited Ca2+ transients as well as the functional characterization of nAChR channels. Unitary acetylcholine-evoked events of alpha 8 nAChR had a slope conductance of 23 pS, whereas two conductance classes (19-23 and 32-45 pS) were identified for all other nAChR channels. The mean channel open times were significantly longer for homomeric alpha 7 and alpha 8 nAChRs (6-7 ms) than for heteromeric nAChRs (1-3 ms), with the exception of alpha 3 beta 4 nAChRs (8.4 ms for rat, 7 ms for chick). At least two species of heterologously expressed nAChRs (alpha 3 beta 4 and alpha 3 beta 2) exhibited single-channel characteristics similar to those reported for native receptors. The variety of nAChR channel conductance and kinetic properties encountered in human cells transfected with nAChR subunits contributes to the functional diversity of nAChRs in nerve cells.
1997
01 Pubblicazione su rivista::01a Articolo in rivista
Functional properties of neuronal nicotinic acetylcholine receptor-channels expressed in transfected human cells / Ragozzino, Davide Antonio; Fucile, Sergio; Giovannelli, A.; Grassi, Francesca; Mileo, A. M.; Ballivet, M.; Alemà, S.; Eusebi, Fabrizio. - In: EUROPEAN JOURNAL OF NEUROSCIENCE. - ISSN 0953-816X. - STAMPA. - 9:(1997), pp. 480-488. [10.1111/j.1460-9568.1997.tb01625.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/242187
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