We present a study of x-ray synchrotron radiation and neutron reflectivity on solid-supported lipid membranes prepared by spin coating. This technique has the advantage of allowing the control of the number of lipid layers by varying the deposition parameters. The experiments were performed on the cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP), the neutral lipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the lipid mixture (DOTAP-DOPC), and the complex (DOTAP-DOPC∕DNA) deposited on wafers. Only single neutral lipids or lipid-peptide mixtures were deposited on solid substrate using the spin coating technique and characterized. Results on the structure of the deposited lipid layers indicate that DNA contributes to the order in the lipoplexes.
X-ray and neutron reflectivity study of solid supported lipid membranes prepared by spin coating / J., Generosi; C., Castellano; R., Felici; G., Fragneto; F., Natali; Pozzi, Daniela; Congiu, Agostina. - In: JOURNAL OF APPLIED PHYSICS. - ISSN 0021-8979. - 96:(2004), pp. 6839-6844. [10.1063/1.1814412]
X-ray and neutron reflectivity study of solid supported lipid membranes prepared by spin coating
POZZI, DANIELA;CONGIU, Agostina
2004
Abstract
We present a study of x-ray synchrotron radiation and neutron reflectivity on solid-supported lipid membranes prepared by spin coating. This technique has the advantage of allowing the control of the number of lipid layers by varying the deposition parameters. The experiments were performed on the cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP), the neutral lipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the lipid mixture (DOTAP-DOPC), and the complex (DOTAP-DOPC∕DNA) deposited on wafers. Only single neutral lipids or lipid-peptide mixtures were deposited on solid substrate using the spin coating technique and characterized. Results on the structure of the deposited lipid layers indicate that DNA contributes to the order in the lipoplexes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
