Heterochromatin protein 1 (HP1), a gene silencing protein, localizes to centric heterochromatin through an interaction with methylated K9 of histone H3, a modification generated by the histone methyl transferase SU(VAR)3-9. On Drosophila polytene chromosomes, HP1 also localizes to 200 sites scattered throughout euchromatin. To address the role of HP1 in euchromatic gene regulation, mRNAs from wild-type and Su(var)2-5 mutants lacking HP1 were compared. Genes residing within a 550-kb genomic region enriched in HP1 that show altered expression in the Su(var)2-5 mutant were analyzed in detail. Three genes within this region, Pros35, CG5676, and cdc2, were found to associate with HP1 by chromatin immunoprecipitation. Surprisingly, these genes require HP1 for expression, suggesting a positive role for HP1 in euchromatic gene expression. Of these genes, only cdc2 is packaged with methylated K9 H3. Furthermore, none of the genes show altered expression in a Su(var)3-9 mutant. Collectively, these data demonstrate multiple mechanisms for HP1 localization within euchromatin and show that some genes associated with HP1 are not affected by alterations in Su(var)3-9 dosage. © 2005 Wiley-Liss, Inc.

Role of Drosophila HP1 in euchromatic gene expression / Diane E., Cryderman; S. H., Grade; Yuhong, Li; Fanti, Laura; Pimpinelli, Sergio; Lori L., Wallrath. - In: DEVELOPMENTAL DYNAMICS. - ISSN 1058-8388. - STAMPA. - 232:3(2005), pp. 767-774. [10.1002/dvdy.20310]

Role of Drosophila HP1 in euchromatic gene expression

FANTI, Laura;PIMPINELLI, Sergio;
2005

Abstract

Heterochromatin protein 1 (HP1), a gene silencing protein, localizes to centric heterochromatin through an interaction with methylated K9 of histone H3, a modification generated by the histone methyl transferase SU(VAR)3-9. On Drosophila polytene chromosomes, HP1 also localizes to 200 sites scattered throughout euchromatin. To address the role of HP1 in euchromatic gene regulation, mRNAs from wild-type and Su(var)2-5 mutants lacking HP1 were compared. Genes residing within a 550-kb genomic region enriched in HP1 that show altered expression in the Su(var)2-5 mutant were analyzed in detail. Three genes within this region, Pros35, CG5676, and cdc2, were found to associate with HP1 by chromatin immunoprecipitation. Surprisingly, these genes require HP1 for expression, suggesting a positive role for HP1 in euchromatic gene expression. Of these genes, only cdc2 is packaged with methylated K9 H3. Furthermore, none of the genes show altered expression in a Su(var)3-9 mutant. Collectively, these data demonstrate multiple mechanisms for HP1 localization within euchromatin and show that some genes associated with HP1 are not affected by alterations in Su(var)3-9 dosage. © 2005 Wiley-Liss, Inc.
2005
chromatin structure; drosophila; epigenetics; gene expression; heterochromatin protein 1; hp1
01 Pubblicazione su rivista::01a Articolo in rivista
Role of Drosophila HP1 in euchromatic gene expression / Diane E., Cryderman; S. H., Grade; Yuhong, Li; Fanti, Laura; Pimpinelli, Sergio; Lori L., Wallrath. - In: DEVELOPMENTAL DYNAMICS. - ISSN 1058-8388. - STAMPA. - 232:3(2005), pp. 767-774. [10.1002/dvdy.20310]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/238278
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