In this letter, we show the results achieved using a hydrogenated amorphous silicon photosensor for a “label-free” deoxyribonucleic acid DNA analysis based on the measurements of the absorbance in the ultraviolet range. The optimization of the sensor structure allowed us to bring the detection limit for a 30-mer DNA sample down to 1 nMcm, limited by the experimental setup. Taking advantage of the hypochromic effect, we also demonstrated the detection of single- and double-stranded DNA molecules in a melting experiment. From the noise characterization of our setup, we estimated the minimum DNA absorbance required to detect the occurrence of a hybridization/separation process to be below 10?3.
Hydrogenated amorphous silicon ultraviolet sensor for deoxyribonucleic acid analysis / DE CESARE, Giampiero; Caputo, Domenico; Nascetti, Augusto; Guiducci, C; Riccò, B.. - In: APPLIED PHYSICS LETTERS. - ISSN 0003-6951. - 88:(2006), pp. 083904-1-083904-3. [10.1063/1.2179608]
Hydrogenated amorphous silicon ultraviolet sensor for deoxyribonucleic acid analysis
DE CESARE, Giampiero;CAPUTO, Domenico;NASCETTI, Augusto;
2006
Abstract
In this letter, we show the results achieved using a hydrogenated amorphous silicon photosensor for a “label-free” deoxyribonucleic acid DNA analysis based on the measurements of the absorbance in the ultraviolet range. The optimization of the sensor structure allowed us to bring the detection limit for a 30-mer DNA sample down to 1 nMcm, limited by the experimental setup. Taking advantage of the hypochromic effect, we also demonstrated the detection of single- and double-stranded DNA molecules in a melting experiment. From the noise characterization of our setup, we estimated the minimum DNA absorbance required to detect the occurrence of a hybridization/separation process to be below 10?3.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
