We present the results of anelastic spectroscopy on the dynamic properties of lipid complexes widely used as DNA vectors in gene therapy. In particular we studied the cationic/neutral lipid mixtures DOTAP/DOPE, DOTAP/DMPC and the single lipids, DOTAP (di-oleoyl trimethylammonium propane), DOPE (di-oleoyl phosphatidylethanolamine), DMPC (dimyristoyl phosphatidylcholine) and DDAB (dimethyldioctadecylammonium), deposited on a solid silicon(1 0 0) substrate. Two relaxation processes have been observed at about 200 and 140 K in mixtures of cationic and neutral lipids, which are due to a dynamics in the membrane plane on time scales accessible to anelastic spectroscopy. The presence of the two processes appears to be a general phenomenon independent of the lipid type, and is connected to the motion of lipids and lipid domains in the membrane plane.
Anelastic spectroscopy as a probe of dynamic properties in lipid membranes / C., Castellano; J., Generosi; Pozzi, Daniela; Cantelli, Rosario. - In: MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING. - ISSN 0921-5093. - 442:1-2 SPEC. ISS.(2006), pp. 375-378. (Intervento presentato al convegno 14th International Conference on Internal Friction and Mechanical Spectroscopy (ICIFMS-14) tenutosi a Kyoto, JAPAN nel SEP 05-09, 2005) [10.1016/j.msea.2006.02.225].
Anelastic spectroscopy as a probe of dynamic properties in lipid membranes
POZZI, DANIELA;CANTELLI, Rosario
2006
Abstract
We present the results of anelastic spectroscopy on the dynamic properties of lipid complexes widely used as DNA vectors in gene therapy. In particular we studied the cationic/neutral lipid mixtures DOTAP/DOPE, DOTAP/DMPC and the single lipids, DOTAP (di-oleoyl trimethylammonium propane), DOPE (di-oleoyl phosphatidylethanolamine), DMPC (dimyristoyl phosphatidylcholine) and DDAB (dimethyldioctadecylammonium), deposited on a solid silicon(1 0 0) substrate. Two relaxation processes have been observed at about 200 and 140 K in mixtures of cationic and neutral lipids, which are due to a dynamics in the membrane plane on time scales accessible to anelastic spectroscopy. The presence of the two processes appears to be a general phenomenon independent of the lipid type, and is connected to the motion of lipids and lipid domains in the membrane plane.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
