Effect of lipid hydration on the structure of lamellar lipoplexes made of the cationic lipid 3-[N-(N,N-dimethylaminoethane)-carbam- oyl]cholesterol (DC-Chol), the neutral ‘helper’ lipid dioleoylphosphatidylethanolamine (DOPE) and calf-thymus DNA was investigated by synchrotron small angle X-ray diffraction (SAXD). Here, we show that lipid hydration is the key factor regulating the equilibrium structure of lipoplexes. Thermotropic behavior was also investigated between 5 and 65 °C. Both the membrane thickness and the water layer thickness were found to decrease linearly as a function of temperature while the one dimensional DNA rod lattice between lipid bilayers was found to enlarge. Structural results were interpreted in terms of recently proposed theoretical models.
How lipid hydration and temperature affect the structure of DC-Chol-DOPE/DNA lipoplexes / Pozzi, Daniela; Heinz, Amenitsch; Caminiti, Ruggero; Caracciolo, Giulio. - In: CHEMICAL PHYSICS LETTERS. - ISSN 0009-2614. - 422:(2006), pp. 439-445. [10.1016/j.cplett.2006.02.106]
How lipid hydration and temperature affect the structure of DC-Chol-DOPE/DNA lipoplexes
POZZI, DANIELA;CAMINITI, Ruggero;CARACCIOLO, Giulio
2006
Abstract
Effect of lipid hydration on the structure of lamellar lipoplexes made of the cationic lipid 3-[N-(N,N-dimethylaminoethane)-carbam- oyl]cholesterol (DC-Chol), the neutral ‘helper’ lipid dioleoylphosphatidylethanolamine (DOPE) and calf-thymus DNA was investigated by synchrotron small angle X-ray diffraction (SAXD). Here, we show that lipid hydration is the key factor regulating the equilibrium structure of lipoplexes. Thermotropic behavior was also investigated between 5 and 65 °C. Both the membrane thickness and the water layer thickness were found to decrease linearly as a function of temperature while the one dimensional DNA rod lattice between lipid bilayers was found to enlarge. Structural results were interpreted in terms of recently proposed theoretical models.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
