We present a simple theoretical analysis of the DNA-DNA electrostatic interactions within charge-neutral lamellar cationic lipid/ DNA complexes (lipoplexes). Although always repulsive as a function of the DNA-DNA interaxial distance, the calculated electrostatic force shows a deep minimum for each value of lipid composition corresponding to an equilibrium distance of the system. The excellent agreement between the equilibrium distances predicted by the model and that experimentally observed in charge-neutral complexes as revealed by synchrotron X-ray diffraction, shows that the spatial dimensionality of both the lipids and the DNA may not be a crucial point to capture the essence of the DNA-DNA interactions within charge-neutral lipoplexes
DNA-DNA electrostatic interactions within cationic lipid/DNA lamellar complexes / Caracciolo, Giulio; Caminiti, Ruggero. - In: CHEMICAL PHYSICS LETTERS. - ISSN 0009-2614. - STAMPA. - 400:(2004), pp. 314-319. [10.1016/j.cplett.2004.10.134]
DNA-DNA electrostatic interactions within cationic lipid/DNA lamellar complexes
CARACCIOLO, Giulio;CAMINITI, Ruggero
2004
Abstract
We present a simple theoretical analysis of the DNA-DNA electrostatic interactions within charge-neutral lamellar cationic lipid/ DNA complexes (lipoplexes). Although always repulsive as a function of the DNA-DNA interaxial distance, the calculated electrostatic force shows a deep minimum for each value of lipid composition corresponding to an equilibrium distance of the system. The excellent agreement between the equilibrium distances predicted by the model and that experimentally observed in charge-neutral complexes as revealed by synchrotron X-ray diffraction, shows that the spatial dimensionality of both the lipids and the DNA may not be a crucial point to capture the essence of the DNA-DNA interactions within charge-neutral lipoplexesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
