Trehalose is a disaccharide important in foods, serving as a glucose source in many and also as an additive in the food preparation. Because of its peculiar physico-chemical properties it plays an important role as preservative in drying and deep-freezing treatments. A new biosensor for trehalose determination has been realized by means of a flow system, based on a reactor in which the trehalase enzyme catalyses its hydrolysis into two ,d-glucose molecules, and a GOD (glucose oxidase) amperometric biosensor is employed for the glucose determination. The optimum operative conditions have been laid out and a particular attention has been paid to the immobilization procedure of the two enzymes. The electrode used is of the SPE (screen-printed electrode) type and has been activated with the Prussian Blue (PB) and then assembled using GOD immobilized with Nafion. The reactor has been prepared with the trehalase enzyme chemically immobilized on an Immunodyne ABC membrane. Asdemonstration of its utility, the biosensor has been testedon a realsample of Boletus edulismushroom.
Construction,assembling and application of a trehalase-GOD enzyme electrode system / Antonelli, Marta Letizia; F., Arduini; Lagana', Aldo; D., Moscone; V., Siliprandi. - In: BIOSENSORS & BIOELECTRONICS. - ISSN 0956-5663. - STAMPA. - 24(5):5(2009), pp. 1382-1388. [10.1016/j.bios.2008.07.084]
Construction,assembling and application of a trehalase-GOD enzyme electrode system
ANTONELLI, Marta Letizia;LAGANA', Aldo;
2009
Abstract
Trehalose is a disaccharide important in foods, serving as a glucose source in many and also as an additive in the food preparation. Because of its peculiar physico-chemical properties it plays an important role as preservative in drying and deep-freezing treatments. A new biosensor for trehalose determination has been realized by means of a flow system, based on a reactor in which the trehalase enzyme catalyses its hydrolysis into two ,d-glucose molecules, and a GOD (glucose oxidase) amperometric biosensor is employed for the glucose determination. The optimum operative conditions have been laid out and a particular attention has been paid to the immobilization procedure of the two enzymes. The electrode used is of the SPE (screen-printed electrode) type and has been activated with the Prussian Blue (PB) and then assembled using GOD immobilized with Nafion. The reactor has been prepared with the trehalase enzyme chemically immobilized on an Immunodyne ABC membrane. Asdemonstration of its utility, the biosensor has been testedon a realsample of Boletus edulismushroom.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
