Abstract: Pentose-borate 1:1 complexes were generated in the ESI source of a triple quadrupole and ion trap mass spectrometer by electrospray ionization of Na2B4O7 and pentose(arabinose, lyxose, ribose,xylose) 2:1 solution in CH3CN/H2O. The study of their low-energy collisionally activated dissociation (CAD) demonstrated that ribose and lyxose are preferentially complexed at the C-2-C-3 cis-diol function whereas arabinose and xylose are esterified at the C-1-C-2 hydroxyl groups. No evidence was found of the stronger affinity for ribose to borate. The ribose probiotic rule can be explained by considering its peculiar capability, among the investigated pentoses, to almost totally complex the borate anion at the C-2-C-3 hydroxyl group, thus enabling the subsequent stages of nucleotide assembly, such as phosphorylation and linkage to the nucleobases. Finally, the differences observed in the pentose-borate complex CAD spectra can be used for the mass spectrometric discrimination of isom...

Low-energy collisionally activated dissociation of pentose-borate complexes / Pepi, Federico; Garzoli, Stefania; Tata, Alessandra; Giacomello, Pierluigi. - In: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY. - ISSN 1387-3806. - STAMPA. - 289:2-3(2010), pp. 76-83. [10.1016/j.ijms.2009.09.012]

Low-energy collisionally activated dissociation of pentose-borate complexes

PEPI, Federico;GARZOLI, STEFANIA;TATA, ALESSANDRA;GIACOMELLO, Pierluigi
2010

Abstract

Abstract: Pentose-borate 1:1 complexes were generated in the ESI source of a triple quadrupole and ion trap mass spectrometer by electrospray ionization of Na2B4O7 and pentose(arabinose, lyxose, ribose,xylose) 2:1 solution in CH3CN/H2O. The study of their low-energy collisionally activated dissociation (CAD) demonstrated that ribose and lyxose are preferentially complexed at the C-2-C-3 cis-diol function whereas arabinose and xylose are esterified at the C-1-C-2 hydroxyl groups. No evidence was found of the stronger affinity for ribose to borate. The ribose probiotic rule can be explained by considering its peculiar capability, among the investigated pentoses, to almost totally complex the borate anion at the C-2-C-3 hydroxyl group, thus enabling the subsequent stages of nucleotide assembly, such as phosphorylation and linkage to the nucleobases. Finally, the differences observed in the pentose-borate complex CAD spectra can be used for the mass spectrometric discrimination of isom...
2010
01 Pubblicazione su rivista::01a Articolo in rivista
Low-energy collisionally activated dissociation of pentose-borate complexes / Pepi, Federico; Garzoli, Stefania; Tata, Alessandra; Giacomello, Pierluigi. - In: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY. - ISSN 1387-3806. - STAMPA. - 289:2-3(2010), pp. 76-83. [10.1016/j.ijms.2009.09.012]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/226466
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