Listeria monocytogenes is a notably invasive bacterium associated with life-threatening food-borne disease in humans. Several surface proteins have been shown to be essential in the adhesion of L. monocytogenes, and in the subsequent invasion of phagocytes. Because the control of the invasion of host cells by Listeria could potentially hinder its spread in the infected host, we have examined the effects of a protease treatment on the ability of L. monocytogenes to form biofilms and to invade tissues. We have chosen serratiopeptidase (SPEP), an extracellular metalloprotease produced by Serratia marcescens that is already widely used as an anti-inflammatory agent, and has been shown to modulate adhesin expression and to induce antibiotic sensitivity in other bacteria. Treatment of L. monocytogenes with sublethal concentrations of SPEP reduced their ability to form biofilms and to invade host cells. Zymograms of the treated cells revealed that Ami4b autolysin, internalinB, and ActA were sharply reduced. These cell-surface proteins are known to function as ligands in the interaction between these bacteria and their host cells, and our data suggest that treatment with this natural enzyme may provide a useful tool in the prevention of the initial adhesion of L. monocytogenes to the human gut.

Protease treatment affects both invasion ability and biofilm formation in Listeria monocytogenes / Longhi, Catia; A., Carpentieri; Seganti, Lucilla; P., Pucci; A., Amoresano; Ps, Cocconcelli; Scoarughi, Gian Luca; Poggiali, Federica; Cellini, Andrea; Artini, Marco; Costerton, JOHN WILLIAM; Selan, Laura. - In: MICROBIAL PATHOGENESIS. - ISSN 0882-4010. - STAMPA. - 45:1(2008), pp. 45-52. [10.1016/j.micpath.2008.01.007]

Protease treatment affects both invasion ability and biofilm formation in Listeria monocytogenes

LONGHI, Catia;SEGANTI, Lucilla;SCOARUGHI, Gian Luca;POGGIALI, Federica;CELLINI, ANDREA;ARTINI, Marco;SELAN, Laura
2008

Abstract

Listeria monocytogenes is a notably invasive bacterium associated with life-threatening food-borne disease in humans. Several surface proteins have been shown to be essential in the adhesion of L. monocytogenes, and in the subsequent invasion of phagocytes. Because the control of the invasion of host cells by Listeria could potentially hinder its spread in the infected host, we have examined the effects of a protease treatment on the ability of L. monocytogenes to form biofilms and to invade tissues. We have chosen serratiopeptidase (SPEP), an extracellular metalloprotease produced by Serratia marcescens that is already widely used as an anti-inflammatory agent, and has been shown to modulate adhesin expression and to induce antibiotic sensitivity in other bacteria. Treatment of L. monocytogenes with sublethal concentrations of SPEP reduced their ability to form biofilms and to invade host cells. Zymograms of the treated cells revealed that Ami4b autolysin, internalinB, and ActA were sharply reduced. These cell-surface proteins are known to function as ligands in the interaction between these bacteria and their host cells, and our data suggest that treatment with this natural enzyme may provide a useful tool in the prevention of the initial adhesion of L. monocytogenes to the human gut.
2008
serratiopeptidase; serrapeptase; listeria monocytogenes virulence; autolysin; adhesin; biofilm
01 Pubblicazione su rivista::01a Articolo in rivista
Protease treatment affects both invasion ability and biofilm formation in Listeria monocytogenes / Longhi, Catia; A., Carpentieri; Seganti, Lucilla; P., Pucci; A., Amoresano; Ps, Cocconcelli; Scoarughi, Gian Luca; Poggiali, Federica; Cellini, Andrea; Artini, Marco; Costerton, JOHN WILLIAM; Selan, Laura. - In: MICROBIAL PATHOGENESIS. - ISSN 0882-4010. - STAMPA. - 45:1(2008), pp. 45-52. [10.1016/j.micpath.2008.01.007]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/225970
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