In this research the production of polyhydroxyalkanoates (PHA) by activated sludge enriched in a sequencing batch reactor (SBR) at high organic load, has been investigated. The SBR was operated at four different organic load rates (OLR), in the range 8.5 - 40.8 gCOD/L/day, and hydraulic retention times (HRT) in the range 1 - 0.21 day, both being simultaneously varied by changing the cycle length from 2 h to 0.42 h. Both parameters affected the establishment of the feast and famine conditions needed for the selection of PHA-producing microorganisms. The highest observed values of polymer production rates and yields (∼ 400 mgCOD/gCOD/h and 0.53 COD/COD, respectively) were obtained at the lowest OLR investigated (8.5 gCOD/L/day), with a corresponding HRT of 1 day. Microbial community analysis, based on denaturing gradient gel electrophoresis (DGGE), revealed that hydraulic and organic loads also played a main role on the microbial speciation within the SBR. Copyright © 2010, AIDIC Servizi S.r.l.
Effect of hydraulic and organic loads in Sequencing Batch Reactor on microbial ecology of activated sludge and storage of polyhydroxyalkanoates / Villano, Marianna; Lampis, Silvia; Valentino, Francesco; Vallini, Giovanni; Majone, Mauro; Beccari, Mario. - In: CHEMICAL ENGINEERING TRANSACTIONS. - ISSN 2283-9216. - STAMPA. - 20:(2010), pp. 187-192. (Intervento presentato al convegno 2nd International Conference on Industrial Biotechnology IBIC2010 tenutosi a Padua, ITALY nel APR 11-14, 2010) [10.3303/cet1020032].
Effect of hydraulic and organic loads in Sequencing Batch Reactor on microbial ecology of activated sludge and storage of polyhydroxyalkanoates
Villano, Marianna;Valentino, Francesco;Majone, Mauro;Beccari, Mario
2010
Abstract
In this research the production of polyhydroxyalkanoates (PHA) by activated sludge enriched in a sequencing batch reactor (SBR) at high organic load, has been investigated. The SBR was operated at four different organic load rates (OLR), in the range 8.5 - 40.8 gCOD/L/day, and hydraulic retention times (HRT) in the range 1 - 0.21 day, both being simultaneously varied by changing the cycle length from 2 h to 0.42 h. Both parameters affected the establishment of the feast and famine conditions needed for the selection of PHA-producing microorganisms. The highest observed values of polymer production rates and yields (∼ 400 mgCOD/gCOD/h and 0.53 COD/COD, respectively) were obtained at the lowest OLR investigated (8.5 gCOD/L/day), with a corresponding HRT of 1 day. Microbial community analysis, based on denaturing gradient gel electrophoresis (DGGE), revealed that hydraulic and organic loads also played a main role on the microbial speciation within the SBR. Copyright © 2010, AIDIC Servizi S.r.l.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
