Abstract: Aqueous and organic extracts of peripheral human T Iymphocytes and of T lymphoblastoid cell lines have been examinated by P-31 and H-1 NMR spectroscopy in order to study the metabolism of ethanolamine (Etn) linked phosphoglycerides. The results show that the Etn concentration in the culture medium determines the composition of Etn-containing metabolites and phospholipids. The effect of phorbol esters, stimulating the synthesis and the breakdown of choline-containing phospholipids has been also studied. A phorbol 12-myristate 13-acetate (PMA) dependent membrane phosphatidylethanolamine hydrolysis, presumably mediated by protein kinase C activity, has been demonstrated.
P-31 and H-1 studies of ethanolamine-linked phosphoglycerides metabolism in human T Lymphocites / R., Ricciolini; Miccheli, Alfredo; G., Peluso; Delfini, Maurizio; Conti, Filippo. - In: CELLULAR AND MOLECULAR BIOLOGY. - ISSN 0145-5680. - STAMPA. - 37:7(1991), pp. 705-711.
P-31 and H-1 studies of ethanolamine-linked phosphoglycerides metabolism in human T Lymphocites
MICCHELI, Alfredo;DELFINI, Maurizio;CONTI, Filippo
1991
Abstract
Abstract: Aqueous and organic extracts of peripheral human T Iymphocytes and of T lymphoblastoid cell lines have been examinated by P-31 and H-1 NMR spectroscopy in order to study the metabolism of ethanolamine (Etn) linked phosphoglycerides. The results show that the Etn concentration in the culture medium determines the composition of Etn-containing metabolites and phospholipids. The effect of phorbol esters, stimulating the synthesis and the breakdown of choline-containing phospholipids has been also studied. A phorbol 12-myristate 13-acetate (PMA) dependent membrane phosphatidylethanolamine hydrolysis, presumably mediated by protein kinase C activity, has been demonstrated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
